We narrowed to 3,806 results for: golden gate

1. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

   Plasmid

   #182354

   Purpose

   Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

   Plasmid

   #182356

   Purpose

   Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMiNGo11-3

   Plasmid

   #126058

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11x3 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMiNGo11-7

   Plasmid

   #126057

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11x7 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL11061

   Plasmid

   #68255

   Purpose

   Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter_AtU6-26 + sgRNA_BolC.GA4a-1
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_H1-g3

   Plasmid

   #247169

   Purpose

   U6 driven gRNA expression of H1-g3 for targeting Dn29 attH1

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   H1-g3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. L4276 hproTGFb1 in pcDNA backbone

   Plasmid

   #244183

   Purpose

   Constitutive expression of human pro-TGF-beta1 (with its signal sequence)

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   pro-TGF-beta1 (TGFB1 Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. L4869 hVEGFA-121 in pcDNA backbone

   Plasmid

   #244180

   Purpose

   Constitutive expression of human VEGFA-121 isoform

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   VEGFA-121 (VEGFA Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. L4868 hVEGFA-165 in pcDNA backbone

   Plasmid

   #244179

   Purpose

   Constitutive expression of human VEGFA-165 isoform

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   VEGFA-165 (VEGFA Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. L4300 hIL10 in pcDNA backbone

    Plasmid

    #244185

    Purpose

    Constitutive expression of human IL-10 (with its signal sequence)

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    IL-10 (IL10 Human, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP1012

    Plasmid

    #239934

    Purpose

    Csy4 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    Csy4
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTKM32

    Plasmid

    #233504

    Purpose

    Level 0 plasmid for MoClo, encodes inducible PGK1-tetO2p for K. marxianus

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Insert

    PGK1-tetO2p
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    addition of two tetracycline operator sequeneces

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

    Plasmid

    #235326

    Purpose

    PiggyBac integration vector for ComMAND open-loop circuit regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235318

    Purpose

    Lentiviral expression of mRuby2 with 3' microRNA only

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

    Plasmid

    #235327

    Purpose

    PiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235268

    Purpose

    Lentiviral expression of ComMAND base gene regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235317

    Purpose

    Lentiviral expression of mRuby2 with intronic microRNA only

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Reckleen_3_multieditdropout

    Plasmid

    #233458

    Purpose

    Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

    Depositor

    Anke Becker

    Article

    Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

    Insert

    Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
    

    Use

    CRISPR

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMDC32B-B/c

    Plasmid

    #227963

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHIE324-ZF6x12-C DsRed-Express2 in TUPV1

    Plasmid

    #201536

    Purpose

    DsRed-Express2 reporter regulated by ZF6 synTF promoter (12x compact binding sites) in TUPV1 for mMoClo golden gate-based assembly

    Depositor

    Joshua Leonard

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    DsRed-Express2
    

    Expression

    Mammalian

    Promoter

    ZF6x12(C)_YB_TATA Minimal Promoter

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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