We narrowed to 10,229 results for: Coli
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Plasmid#217368PurposeExpresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
UseAAVExpressionMammalianMutationC2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83CPromoterU6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N-term D-N/E-Q
Plasmid#233095PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)DepositorInsertYihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
TagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to asparagine (…Available SinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-N-term D-A/E-L
Plasmid#233097PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)DepositorInsertYihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
TagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to alanine (A) …Available SinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-Ec.coaD (pESC106)
Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaE (pESC124)
Plasmid#50390PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertDPCK
Tags6XHIS tagExpressionBacterialPromoterT7Available SinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
ExpressionBacterialMutationH840A of Cas9Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx2
Plasmid#51559PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)DepositorInsertsuperfolder GFP
TagsGLESTCRHASLAVLADERRFSA and MARARAExpressionBacterialMutationContains additonal aa in the N and C terminus (in…Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
TagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA
Plasmid#64983PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSIG1
Plasmid#226908PurposeMoClo-compatible vectors for dsRNA production in E. coliDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7 promoterAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only