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Showing: 6641 - 6680 of 8645 results

  1. pGEM_Δflv3.sm

    Plasmid
    #185533
    Purpose
    a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    aadA gene flanked by flv3 upstream and downstream regions
    Use
    Synthetic Biology
    Available Since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185533-plasmid-map-sequence-id-371409

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  2. psiT-GEM(0)

    Plasmid
    #220446
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Expression
    Insect
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220446-plasmid-map-sequence-id-443761

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  7. psiT-GEM(2)

    Plasmid
    #220448
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Expression
    Insect
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220448-plasmid-map-sequence-id-438388

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  8. psiT-GEM(1)

    Plasmid
    #220447
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Expression
    Insect
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220447-plasmid-map-sequence-id-436669

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  13. Rif1-Halo HRD

    Plasmid
    #207081
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    None
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207081-plasmid-map-sequence-id-432324

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  14. RNF169-Halo HRD

    Plasmid
    #207083
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207083-plasmid-map-sequence-id-432319

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  17. Halo-SHLD2 HRD

    Plasmid
    #207078
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    207078-plasmid-map-sequence-id-431782

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  19. SNAP-LC3 HRD

    Plasmid
    #207545
    Purpose
    Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
    Tags
    SNAPtag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207545-plasmid-map-sequence-id-430987

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  20. Halo-LC3B HRD

    Plasmid
    #207539
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    207539-plasmid-map-sequence-id-429514

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  23. INT_Cargo_T7_gfp

    Plasmid
    #212136
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Article
    Fung et al Biomaterials. 2022 Aug;287:121661. doi:
    Insert
    green fluorescent protein
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212136-plasmid-map-sequence-id-420767

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  36. NK809

    Plasmid
    #196406
    Purpose
    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Mutation
    N/A (synthetic)
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196406-plasmid-map-sequence-id-385017

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  37. NK810

    Plasmid
    #196407
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Mutation
    N/A (synthetic)
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196407-plasmid-map-sequence-id-385018

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  38. NK811

    Plasmid
    #196408
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Mutation
    N/A (synthetic)
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196408-plasmid-map-sequence-id-384995

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  40. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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Showing: 6641 - 6680 of 8645 results