We narrowed to 9,406 results for: Coli

1. PD-1-miSFIT-12-C, 21-G

   Plasmid

   #124683

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PD-1-miSFIT-1-A, 5-A

   Plasmid

   #124682

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. yTREX-ApR

   Plasmid

   #177307

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYTRW21K_1Ti1

   Plasmid

   #177291

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and eYFP

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   eYFP
   

   Use

   Synthetic Biology; Yeast expression, rrn genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYTSK56K_3G7

   Plasmid

   #177301

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherry

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   mCherry
   

   Use

   Synthetic Biology; Yeast expression, tn7 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYTSK58K_6G7

   Plasmid

   #177302

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   pe-h
   

   Use

   Synthetic Biology; Yeast expression, tn7 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYTRW15K_2G5

   Plasmid

   #177284

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mTagBFP2

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   mTagBFP2
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYTRW17K_6G5

   Plasmid

   #177285

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and PE-H

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   pe-h
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYTRW16K_1G5

   Plasmid

   #177286

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and eYFP

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   eYFP
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYTRW13K_3G5

    Plasmid

    #177287

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mCherry

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    mCherry
    

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYTRW18K_3T5

    Plasmid

    #177288

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR and mCherry

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    mCherry
    

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYTRW28K_0Ti1

    Plasmid

    #177290

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    PsacB-sacB
    

    Use

    Synthetic Biology; Yeast expression, rrn genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiCRISPR v2-sgWAVE2-2

    Plasmid

    #210136

    Purpose

    knock out WAVE2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Actin-binding protein WASF2 (WASF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPR v2-sgWAVE2-1

    Plasmid

    #210135

    Purpose

    knock out WAVE2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Actin-binding protein WASF2 (WASF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPodA11

    Plasmid

    #162843

    Purpose

    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance

    Depositor

    Dianne Newman

    Article

    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11

    Insert

    Protein Designed Pyocyanin demethylase
    

    Use

    Tags

    TEV-cleavable 6x-His tag

    Expression

    Bacterial

    Mutation

    A53N, I73T, A87V, M99V, A129T from WT PodA

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMCh-607

    Plasmid

    #92258

    Purpose

    Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCh-633

    Plasmid

    #92253

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    EF1A

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYTRW26K_1Ti1

    Plasmid

    #177292

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    PsacB-sacB
    

    Use

    Synthetic Biology; Yeast expression, rrn genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Flag-NSF

    Plasmid

    #74924

    Purpose

    Mammalian expression of human NSF full-length (744 aa)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCas-dCas12m

    Plasmid

    #192275

    Purpose

    Encodes MmdCas12m (D485A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCas-Cas12m

    Plasmid

    #192274

    Purpose

    Encodes MmCas12m under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEGFP-PI35P2

    Plasmid

    #92419

    Purpose

    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

    Insert

    MCOLN1 (Mcoln1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Synthetic gene, codon optimized for human.

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lentiCRISPR v2-sgBAX-1

    Plasmid

    #210013

    Purpose

    knock out BAX in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Apoptosis regulator BAX (BAX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

    Plasmid

    #192822

    Purpose

    Golden Gate compatible plasmid with mCherry and Neo

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    mCherry and Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2-GFP-MKLP1

    Plasmid

    #140566

    Purpose

    Expresses GFP tagged MLKP1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lentiCRISPR v2-sgBAX-2

    Plasmid

    #210130

    Purpose

    knock out BAX in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Apoptosis regulator BAX (BAX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS2-mKate-MKLP1

    Plasmid

    #140570

    Purpose

    Expresses mKate tagged MKLP1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    mKate

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2-mCherry-MKLP1

    Plasmid

    #140562

    Purpose

    Expresses mCherry tagged MKLP1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    2xmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. plko-tet-on-puromycin-shTRX1-259

    Plasmid

    #110920

    Purpose

    To knockdown Thioredoxin-1 following doxycycline addition

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SIN-cPPT-G1B3-mitoGO-ATeam2-WPRE-miR124T

    Plasmid

    #245075

    Purpose

    Mitochondrial ATP biosensor, with miRNA-124 target sequence to repress transgene expression in neurons

    Depositor

    Nicole Déglon

    Article

    Richetin et al Nat Neurosci. 2020 Dec;23(12):1567-1579.

    Insert

    mitoGO-ATeam2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human G1B3 promoter

    Available Since

    Oct. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pYTRW27K_7Ti1

    Plasmid

    #177294

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZ

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    PsacB-sacB
    

    Use

    Synthetic Biology; Yeast expression, rrn genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYTRW07K_0G5

    Plasmid

    #177279

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYTRW08K_0C5

    Plasmid

    #177280

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmR

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    Pcat-cat
    

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYTRW11K_0S5

    Plasmid

    #177282

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with SmR

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    PaadA-aadA
    

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYTRW20K_0Ti1

    Plasmid

    #177289

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    Ptet-tetA(C)
    

    Use

    Synthetic Biology; Yeast expression, rrn genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCas-Cas12m-ΔZF

    Plasmid

    #192276

    Purpose

    Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    Cas12m ΔZF
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    H459A, C552A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pS97splitRBP_S2-Enz

    Plasmid

    #183768

    Purpose

    Expresses SHIP2-Enzyme domain flanked by RBP

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Tags

    6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

    Expression

    Bacterial

    Mutation

    includes aa K414 to aa V872 only

    Promoter

    T7lac

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMCh-623

    Plasmid

    #92266

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 6W6A (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced W23…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMCh-695

    Plasmid

    #92263

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 PAM2 mut (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced: EF…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMCh-660

    Plasmid

    #92259

    Purpose

    Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMCh-665

    Plasmid

    #92254

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    EF1A

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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43. pET24TEV-tSHIP1

    Plasmid

    #183770

    Purpose

    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP1 (INPP5D Human)
    

    Use

    Tags

    6HIS and Tobacco Etch Virus (TEV) Cleavage site

    Expression

    Bacterial

    Mutation

    includes aa1 to aa883 only

    Promoter

    T7 lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. hETFDH CRISPR_1

    Plasmid

    #232309

    Purpose

    Human ETFDH Gene Knockout (gRNA 1)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    ETFDH-targeting gRNA (ETFDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. mEtfdh CRISPR_2

    Plasmid

    #232313

    Purpose

    Mouse Etfdh Gene Knockout (gRNA 2)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh-targeting gRNA (Etfdh Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. mEtfdh CRISPR_1

    Plasmid

    #232312

    Purpose

    Mouse Etfdh Gene Knockout (gRNA 1)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh-targeting gRNA (Etfdh Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. hETFDH CRISPR_2

    Plasmid

    #232310

    Purpose

    Human ETFDH Gene Knockout (gRNA 2)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    ETFDH-targeting gRNA (ETFDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCas-pML-1B_6xHis-Cas12m

    Plasmid

    #192282

    Purpose

    MmCas12m fused to His-tag for protein purification

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12m
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCas-MmdCas12m-CBE1

    Plasmid

    #192284

    Purpose

    MmdCas12m-CBE1 expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m-CDA-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. lentiCRISPR v2-sgBAK-2

    Plasmid

    #210132

    Purpose

    knock out BAK in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Bcl-2 homologous antagonist/killer (BAK1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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