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pCIneo-V5-SBP-MBP-HsNot4_109-189-GSSG-275-575_AG

Plasmid

#148767

Purpose

Mammalian Expression of HsNot4_109-189-GSSG_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_109-189-GSSG_275-575 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-del377-424_AG

Plasmid

#148771

Purpose

Mammalian Expression of HsNot4_275-575-del377-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-424 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-del400-428_AG

Plasmid

#148772

Purpose

Mammalian Expression of HsNot4_275-575-del400-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del400-428 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-F405D_AG

Plasmid

#148773

Purpose

Mammalian Expression of HsNot4_275-575-F405D

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-F405D (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-F408A_AG

Plasmid

#148774

Purpose

Mammalian Expression of HsNot4_275-575-F408A

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-F408A (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-FFTLtoDAEE_AG

Plasmid

#148775

Purpose

Mammalian Expression of HsNot4_275-575-FFTLtoDAEE

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-FFTLtoDAEE (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-L415E_AG

Plasmid

#148776

Purpose

Mammalian Expression of HsNot4_275-575-L415E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-L415E (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-T411E_AG

Plasmid

#148777

Purpose

Mammalian Expression of HsNot4_275-575-T411E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-T411E (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GLRX-roGFP2 in pT3TS-Dest

Plasmid

#194297

Purpose

In vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRK

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

In vitro transcription of mrna

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4-del190-274_AG

Plasmid

#148782

Purpose

Mammalian Expression of HsNot4-del190-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del190-274 (CNOT4 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcryGFP-acta1-dmdGFP

Plasmid

#172530

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.

Depositor

Joachim Berger

Article

Berger et al J Cell Mol Med. 2020 Jun;24(12):6680-668

Insert

dmd (dmd Zebrafish)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pmApple-EGFP-HRas

Plasmid

#133854

Purpose

Mammalian expression of HRas N-terminally tagged with mApple and EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

HRas (HRAS Human)

Tags

EGFP and mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.007

Plasmid

#119671

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Dx2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated downstrea…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.006

Plasmid

#119670

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Ux2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated upstream …

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1

Plasmid

#123326

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2

Plasmid

#123327

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19_T790M_28bp

Plasmid

#114721

Purpose

28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790M_28bp

Plasmid

#114719

Purpose

4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK480] pLJC5 Flag-Depdc5(A)

Plasmid

#109338

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant A

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

DIYGD(185-189)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-A mSesn2 (delBC)

Plasmid

#111797

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

BC domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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