We narrowed to 9,363 results for: BLI

1. lenti-UCOE-dCas9-BFP-VP64

   Plasmid

   #196717

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RABGEF1A-c014

   Plasmid

   #175478

   Purpose

   Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   RABGEF1 (RABGEF1 Human)
   

   Tags

   His6-Zbasic-TEV

   Expression

   Insect

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX404 Campylobacter jejuni Cas9

   Plasmid

   #68338

   Purpose

   Campylobacter jejuni Cas9

   Depositor

   Feng Zhang

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZP06

   Plasmid

   #232320

   Purpose

   pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.

   Depositor

   Chenggang Wu

   Insert

   Pfdx-E
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-MBP-NLS-Geo_st

   Plasmid

   #87703

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-H12TEV

   Plasmid

   #208635

   Purpose

   for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-synp-F-synaptophysin-jRGECO1a

   Plasmid

   #102997

   Purpose

   Expresses synaptophysin fused jRGECO1a under the synapsin promoter

   Depositor

   David Cox

   Insert

   Synaptophysin-jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-mArg1

   Plasmid

   #34573

   Depositor

   Peter Murray

   Insert

   Arg1 (Arg1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQXCINeo-LOXL2-BC

   Plasmid

   #134763

   Purpose

   a retroviral vector to express lysyl oxidaseL2

   Depositor

   Philippe Clezardin, Martine Croset

   Insert

   LOXL2 (LOXL2 )
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PYCR1

    Plasmid

    #38842

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PYCR1 (PYCR1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pMIY-Arg1

    Plasmid

    #34580

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pcDNAI Neo mouse PECAM FL

    Plasmid

    #42901

    Depositor

    Steven Albelda

    Insert

    PECAM (Pecam1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pRS303-PADH1-AFB2

    Plasmid

    #99530

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSP12::dsRed2

    Plasmid

    #30171

    Depositor

    Lalita Ramakrishnan

    Article

    Cosma et al Nat Immunol. 2004 Aug . 5(8):828-35.

    Insert

    Mycobacterium Strong Promoter (MSP)
    

    Use

    Mycobacteria expression

    Tags

    dsRed2

    Available Since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-3'TwStrep

    Plasmid

    #208053

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SNX32A-c014

    Plasmid

    #175486

    Purpose

    Protein expression in bacterial cells. PX domain, S17-M197

    Depositor

    Opher Gileadi

    Insert

    SNX32 (SNX32 Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-5'TwStrep

    Plasmid

    #208620

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-hUCP4-NE

    Plasmid

    #102362

    Purpose

    Overexpression of solute carrier family 25 member 27 in mammalian cells

    Depositor

    Shu Leong Ho

    Insert

    solute carrier family 25 member 27 (SLC25A27 Human)
    

    Tags

    NE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid

    #51656

    Purpose

    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

    Depositor

    Adam Cohen

    Insert

    Quasar2-mOrange-Chr64-GFP
    

    Use

    Lentiviral

    Tags

    GFP and mOrange

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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