We narrowed to 4,461 results for: erf

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pGH125_dCas9-Blast

Plasmid

#85417

Purpose

lentiviral expression vector for dCas9 with Blasticidin selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

dCas9 and Blasticidin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-BsaI_pJMP2776

Plasmid

#208890

Purpose

Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE47

Plasmid

#90385

Purpose

IFNB1 - Interferon beta gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IFNB1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHdzCas9-KRAB

Plasmid

#92341

Purpose

Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

Depositor

I-Son Ng

Article

Kao et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

CRISPR; Plant expression

Tags

KRAB

Expression

Plant

Promoter

CaMV35S

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSR58.6

Plasmid

#63176

Purpose

Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pPM_Pveg-sfgfp

Plasmid

#121503

Purpose

Integration vector that expresses gfp in S. mutans

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

Superfolder GFP

Use

S. mutans integration vector, integrates at the m…

Promoter

Pveg

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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LCAPCW

Plasmid

#205646

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

hCMV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Expression

Bacterial

Promoter

p23

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pM2s2AsG

Plasmid

#137924

Purpose

E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

Superfolder GFP

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23109

Plasmid

#113151

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pWS5398-CRISPRai-HygR

Plasmid

#182837

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCB-CRISPRi

Plasmid

#221136

Purpose

Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

cbu1169 promoter

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEF_dCas9

Plasmid

#68416

Purpose

Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x3

Plasmid

#63597

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.G

Plasmid

#200999

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMT423

Plasmid

#139382

Purpose

Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Constitutively expressed dCas9, LacI and TetR

Use

Synthetic Biology

Promoter

P1

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKEW321-MbCas12a-RFP-GFP-array

Plasmid

#115661

Purpose

Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

GFP and RFP array for MbCas12

Use

CRISPR

Expression

Bacterial

Promoter

Plac

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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