We narrowed to 4,969 results for: mcs

1. sgLATS1/2-2

   Plasmid

   #229432

   Purpose

   knockout of LATS1 and LATS2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgLATS1/2-2 (LATS1, LATS2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIG-829_HA-GD2-28z_CAR_JUN_Retroviral

   Plasmid

   #207506

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   JUN, HA-GD2-28z_CAR (JUN Human)
   

   Use

   Retroviral

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304-ARNT-d414

   Plasmid

   #203579

   Purpose

   Expresses a functionally inactive version of ARNT with 5’ 414 bp deletion [Δ414] in mammalian cells.

   Depositor

   William Kaelin, Abhishek Chakraborty

   Article

   Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

   Insert

   ARNT (ARNT Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   5' 414 basepair deletion [Δ414]

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT259 Lenti pEF-dCas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast

   Plasmid

   #187335

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Cas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgCrebbp#1/Cre

   Plasmid

   #193209

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgCrebbp#1 (Crebbp Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO-PPO-Venus

   Plasmid

   #139505

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV8 and AAV9

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

   Plasmid

   #187320

   Purpose

   dCas9 expression vector with cloning site for C terminal fusions

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIGF1_P1 Promoter-FLuc

   Plasmid

   #154266

   Purpose

   Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   IGF-1 canonical P1 Promoter (IGF1 Human)
   

   Expression

   Mammalian

   Promoter

   Human IGF-1 P1 Promoter

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Myc-CyclinD1(WT)

   Plasmid

   #122300

   Purpose

   expresses Cyclin D1 protein with a Myc tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   CCND1 (CCND1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-DIO-PPO-Venus

    Plasmid

    #139504

    Purpose

    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Has Service

    AAV5

    Insert

    parapinopsin
    

    Use

    AAV

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #187341

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1/PD-L1_ICD

    Plasmid

    #121480

    Purpose

    expression of human PD-L1 in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TM+ICD

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

    Plasmid

    #202059

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
    

    Use

    AAV; Aav with reporter

    Expression

    Mammalian

    Mutation

    ITRs intact

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1/PD-L1_ECD

    Plasmid

    #121479

    Purpose

    expression of human PD-L1 in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ECD+TM

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188747

    Purpose

    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-HA-PD-L1 3SA

    Plasmid

    #121493

    Purpose

    Expression of human PD-L1 3SA in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S176A, T180A, S184A

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

    Plasmid

    #195550

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE

    Plasmid

    #188758

    Purpose

    Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    VP64
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGIPz-PD-L1 4NQ

    Plasmid

    #121487

    Purpose

    Expression of human PD-L1 4NQ with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Inserts

    PD-L1 4NQ
    hPD-L1 shRNA
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N35Q, N192Q, N200Q and N219Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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