We narrowed to 6,371 results for: siae

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pDA186

Plasmid

#74250

Purpose

Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

Inducible peptide of the dPSTR

Expression

Bacterial and Yeast

Promoter

pGPD1

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDA171

Plasmid

#74251

Purpose

Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

Inducible peptide of the dPSTR

Tags

Venus

Expression

Bacterial and Yeast

Promoter

pSTL1

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRS425-M25-Afg1

Plasmid

#61720

Purpose

yeast Afg1 AAA protein in yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

Afg1 (AFG1 Budding Yeast)

Expression

Yeast

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
Gal4pBS13-Pleum

Plasmid

#60332

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

May 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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UASGAL-CU1-Pleum

Plasmid

#60331

Purpose

Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pFA5

Plasmid

#46791

Purpose

To express a version of Fun30 without CUE domain in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 without CUE domain (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only
pFA12

Plasmid

#46792

Purpose

To express a version of Fun30 with a mutated CUE domain in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30, CUE domain mutated (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Mutation

FP82AA

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only
pTH738-CENBEVYslow

Plasmid

#38220

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

GCN4-based single uORF-containing 5'-UTR

Expression

Yeast

Mutation

The start codons of uORFs2, 3 and4 of the yeast G…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLG-Nde-Acc

Plasmid

#33150

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

AccI site in intron was cut/filled/ligated to cha…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pES1004

Plasmid

#21790

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Kss1-as2-trunc (KSS1 Budding Yeast)

Expression

Yeast

Mutation

deleted bases 1-14 from open reading frame E94A …

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A-YFP

Plasmid

#18692

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

Plasmid

#15602

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Budding Yeast, Candida albicans)

Tags

7xHis and Cysteine

Expression

Bacterial

Mutation

4 G/A. Four glycines mutated to alanines. The pos…

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pCpSRb-5

Plasmid

#13727

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

SRβ circularly permuted D210E188 improved solubil…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pPS835

Plasmid

#8933

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

G21V, stabilizes the GTP-bound form

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pJLI-Sup35N-KDG6-C

Plasmid

#1238

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Use

Yeast integrative plasmid

Mutation

SUP35 middle region (aa124-253) was replaced with…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pG1-NMGR526(d22-69)

Plasmid

#1125

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)

Tags

GR526

Expression

Yeast

Mutation

nucleotides encoding NM domain amino acids 22-69 …

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
HDAC5 Flag

Plasmid

#13822

Purpose

Mammalian expression of human histone deacetylase 5 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC5 (HDAC5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pBV163

Plasmid

#108364

Purpose

Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.

Depositor

Scott Moye-Rowley

Article

Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

Insert

Knock-in Cg TDH3 promoter in Cg YAP1 gene

Use

Replace c.glabrata yap1 promoter with c. glabrata…

Promoter

TDH3

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7repeats)-GFP-Pho8

Plasmid

#207011

Purpose

Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

GFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMK419 (GAL-OsTIR1(F74G))

Plasmid

#140656

Purpose

GAL-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74G)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2793

Plasmid

#64043

Purpose

pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter and none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTA-Mob 2.0

Plasmid

#149662

Purpose

Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

Depositor

Bogumil Karas

Article

Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Expression

Bacterial

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCLASP_mScarlet_CLASP_RGS2membrane

Plasmid

#133086

Purpose

Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

mScarlet-CLASP

Expression

Bacterial and Yeast

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJOG1368

Plasmid

#167301

Purpose

Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

FCY-UPP fusion gene

Use

Synthetic Biology; Moclo level 0 vector

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-OSH2-2xPH

Plasmid

#161987

Purpose

Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

Insert

OSH2 (OSH2 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pHLUM

Plasmid

#40276

Depositor

Markus Ralser

Article

Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

Inserts

LEU2 (LEU2 Budding Yeast)
URA3 (URA3 Budding Yeast)
MET15/17 (MET17 Budding Yeast)

Expression

Yeast

Promoter

endogenous LEU2, endogenous MET15/17, and endogen…

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pK036.TRE-Flpe-WPRE (Supernova)

Plasmid

#85007

Purpose

For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

NLS-FLPe-WPRE

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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