Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,945 results for: MLS

Showing: 3341 - 3360 of 4945 results

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pD649-HAsp-ZPBP-Fc(DAPA)-AviTag-6xHis

Plasmid

#156747

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ZPBP (ZPBP Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LTF-COMP5AP-AviTag-9xHis

Plasmid

#157510

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LTF (LTF Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PSG9-COMP5AP-AviTag-9xHis

Plasmid

#157388

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG9 (PSG9 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_2

Plasmid

#195133

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 2 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_1

Plasmid

#195132

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PSG9-Fc(DAPA)-AviTag-6xHis

Plasmid

#156824

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG9 (PSG9 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-LTF-Fc(DAPA)-AviTag-6xHis

Plasmid

#156946

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LTF (LTF Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-WFIKKN1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156905

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

WFIKKN1 (WFIKKN1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-WFIKKN2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156911

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

WFIKKN2 (WFIKKN2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-BPI-Fc(DAPA)-AviTag-6xHis

Plasmid

#156869

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

BPI (BPI Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-A1BG-Fc(DAPA)-AviTag-6xHis

Plasmid

#156875

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

A1BG (A1BG Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-TIE1(FL)-FLAG

Plasmid

#157621

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TIE1 (TIE1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-CCL4-Fc(DAPA)-AviTag-6xHis

Plasmid

#156489

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CCL4 (CCL4 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-IL23A-Fc(DAPA)-AviTag-6xHis

Plasmid

#156566

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IL23A (IL23A Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-LEP-Fc(DAPA)-AviTag-6xHis

Plasmid

#156538

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LEP (LEP Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-anti-MIR328-3p

Plasmid

#153319

Purpose

Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR328-3p sponge

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-anti-MIR211-5p

Plasmid

#153318

Purpose

Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR211-5p sponge

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169318

Purpose

Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

Plasmid

#169316

Purpose

Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.SFFV.mTagBFP2.miR-125b-2

Plasmid

#169311

Purpose

Constitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3341 - 3360 of 4945 results

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