We narrowed to 18,263 results for: Met;

1. Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65253

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Str-KDEL_ST-SBP-mCherry

   Plasmid

   #65265

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)

   Plasmid

   #185801

   Purpose

   one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mRuby2-SiT-N-15

   Plasmid

   #55912

   Purpose

   Localization: Golgi TGN, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   SiT (ST6GAL1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   aa 1-45 of SiT

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pAAVf-EnhCB-lacZnls miR-122 3x

   Plasmid

   #35644

   Depositor

   Phillip Zamore

   Article

   Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

   Insert

   3 miR-122 target sites
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CB

   Available since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-TDsmURFP-IRES-HO-1

   Plasmid

   #80346

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

    Plasmid

    #98925

    Purpose

    AAV expression of Ruby2 spaghetti monster FLAG construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    Ruby2-smFP-Flag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Salsa6f

    Plasmid

    #140188

    Purpose

    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

    Depositor

    Michael Cahalan

    Article

    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

    Insert

    Salsa6f
    

    Use

    Tags

    tdTomato-v5-GCaMP6f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLX303

    Plasmid

    #25897

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-CRY2-mCherry

    Plasmid

    #58368

    Purpose

    Mamlian codon optimized CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mScarlet-MYO10

    Plasmid

    #145179

    Purpose

    Expresses mScarlet-MYO10 in mammalian cells

    Depositor

    Johanna Ivaska

    Article

    Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

    Insert

    mScarlet-MYO10 (MYO10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCF806-shRen.713

    Plasmid

    #186712

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    shRen.713
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid

    #224899

    Purpose

    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

    Depositor

    Thomas Norman

    Article

    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

    Inserts

    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_BFP

    Plasmid

    #25891

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    BFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAVS1-Nst-MCS

    Plasmid

    #80487

    Purpose

    donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSFDuet-1_VNp6-mNeongreen

    Plasmid

    #182388

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge