We narrowed to 4,461 results for: erf

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Expression

Bacterial

Promoter

Ec-TTL-P112 and BBa_J23116

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-6xHis

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRS42H_PTEF1_dCas9_TCYC1

Plasmid

#177155

Purpose

Expresses dCas9 in yeast cells

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

Nuclease-null Cas9

Tags

SV40 NLS

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-AS34a

Plasmid

#37099

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

perfect complementary binding site for hsa-miR-34a (MIR34A Human)

Use

Luciferase

Available Since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pTn7-SCOUT12.G

Plasmid

#201000

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pdCas9_CL

Plasmid

#166245

Purpose

regulated dCas9 generator

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

P112-sgRNA-term-P104-RBS1-dCas9-B15

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ec-TTL-P112 and Ec-TTL-P104

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5397-CRISPRai-NatR

Plasmid

#182836

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJT119b

Plasmid

#50551

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET30-T7-sfGFP

Plasmid

#180757

Purpose

pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5321-CRISPRai-Markerless

Plasmid

#182828

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - markerless

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-RILP

Plasmid

#74600

Purpose

used for expression in E. coli and purification

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab-interacting lysosomal protein (RILP Human)

Tags

FLAG, StrepII, and superfolder GFP

Expression

Bacterial

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMM704

Plasmid

#68891

Purpose

IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMP71-IFN-B

Plasmid

#174597

Purpose

Expresses secreted murine interferon beta

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

secreted IFN-beta

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-NMYC TRF2 deltaB deltaM

Plasmid

#16355

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pSET-dCas9-actII-4-NT-S1

Plasmid

#110185

Purpose

Repression of gene expression in Streptomyces by CRISPRi

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Inserts

dCas9
sgRNA

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

J23119 and ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pP4-C

Plasmid

#225097

Purpose

gfpUV reporter vector with a high performance adenine-dependent riboswitch

Depositor

Robert Batey

Article

Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

Insert

synthetic riboswitch

Expression

Bacterial

Promoter

synthetic constitutive promoter

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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DT037A

Plasmid

#159398

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 (strong) promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x2

Plasmid

#63596

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D1361Y

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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