We narrowed to 16,827 results for: puromycin

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pLenti_C_Myc_DDK_IRES_Puro_TARBP2

Plasmid

#106119

Purpose

Lentiviral expression of TARBP2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TARBP2 (TARBP2 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Puro.let-7g.Cre

Plasmid

#17409

Purpose

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pIRESpuro2 AXL

Plasmid

#65627

Purpose

Expresses AXL on cistronic transcript with puro resistance in mammalian cells

Depositor

Aaron Meyer

Article

Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.

Insert

Tyrosine-protein kinase receptor UFO (AXL Human)

Expression

Mammalian

Mutation

Spurious PDPRPHR on C terminus

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagC shRNA #2

Plasmid

#26630

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagC shRNA (RRAGC Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pBlueScript-humanAPOBEC1

Plasmid

#134606

Purpose

Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

humanAPOBEC1 (APOBEC1 Human)

Expression

Mammalian

Promoter

Beta actin

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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TRAIP-SFB

Plasmid

#101769

Purpose

Mammalian expression of TRAIP fusion protein

Depositor

Michael Huen

Article

Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

Insert

TRAIP / RNF206 (TRAIP Human)

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1 RagA

Plasmid

#73031

Purpose

Expresses human RagA from a CMV promoter for stable expression in mammalian cells

Depositor

David Sabatini

Article

Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

Insert

rragA (RRAGA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-VAMP8

Plasmid

#45919

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
PGK-mCherry

Plasmid

#236119

Purpose

Fluorescent transfection marker encoding mCherry under PGK promoter

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

mCherry

Expression

Mammalian

Promoter

PGK

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagD shRNA #2

Plasmid

#30322

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101104

Purpose

Encodes dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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CAG.CenpB(FL)-GFP-Halo

Plasmid

#176205

Purpose

Centromeric anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

CenpB-GFP-Halo

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgIREB2_1

Plasmid

#169894

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-NLS-LSSmKate2

Plasmid

#34586

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate2

Use

Retroviral

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pPBpuro-4493NES(Booster-PKA)

Plasmid

#184709

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dIDR2)

Plasmid

#192811

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR2

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEM705 Gso (Cys)

Plasmid

#109375

Purpose

GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gso chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NEK1_IDG-K

Plasmid

#189871

Purpose

Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

NEK1 (NEK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1

Plasmid

#221494

Purpose

NKX2-1 short isoform lentiviral overexpression using hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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