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We narrowed to 6,292 results for: nls

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Showing: 5041 - 5070 of 6292 results

  1. AA185

    Plasmid
    #216048
    Purpose
    Fragmid fragment: (C' terminus) for prime editing; using PEmax; for PE4 & 5
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1; P2A_v1.2; MLH1dn_v1.1
    Use
    Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216048-plasmid-map-sequence-id-431510

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  4. Td-CBEmax

    Plasmid
    #196600
    Purpose
    Mammalian expression of Td-CBEmax
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196600-plasmid-map-sequence-id-386793

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  10. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Article
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162277-plasmid-map-sequence-id-319794

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  11. hA3A*-eIscBn-D

    Plasmid
    #227147
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-HMG-D-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227147-plasmid-map-sequence-id-453978

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  12. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  13. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

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  14. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid
    #208952
    Purpose
    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208952-plasmid-map-sequence-id-415741

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  15. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

    Plasmid
    #208960
    Purpose
    A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208960-plasmid-map-sequence-id-415786

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  18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid
    #208953
    Purpose
    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208953-plasmid-map-sequence-id-415742

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  19. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid
    #208955
    Purpose
    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208955-plasmid-map-sequence-id-415744

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  21. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid
    #208954
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208954-plasmid-map-sequence-id-415743

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  23. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

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  24. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid
    #208957
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208957-plasmid-map-sequence-id-415746

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  25. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid
    #208946
    Purpose
    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208946-plasmid-map-sequence-id-415680

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  26. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid
    #217801
    Purpose
    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217801-plasmid-map-sequence-id-432878

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  28. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid
    #208947
    Purpose
    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208947-plasmid-map-sequence-id-415686

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  29. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid
    #208943
    Purpose
    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208943-plasmid-map-sequence-id-415668

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  30. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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Showing: 5041 - 5070 of 6292 results