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pMIRNA1-METTL1-WT

Plasmid

#254424

Purpose

Mammalian expression plasmid encoding human METTL1 fused to a 3_FLAG tag.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

3_FLAG

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shtRNA-Phe(GAA)-2

Plasmid

#254427

Purpose

Mammalian expression plasmid encoding an shRNA targeting tRNA-Phe(GAA).

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

tRNA-Phe (anticodon GAA) 1-4

Use

Lentiviral

Promoter

U6

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-MCS-noATG-GFP

Plasmid

#223181

Purpose

A multicloning site (MCS) was synthesized and inserted into AAV-CMV-GFP to construct the AAV-CMV-MCS-(no ATG)-GFP negative control plasmid.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Type

Empty backbone

Use

AAV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAPwt

Plasmid

#210643

Purpose

Expresses the FAP (Fibroblast Activation Protein) protein.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

none

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBL155c

Plasmid

#246592

Purpose

express cCadC NtRbcL fusion protein

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

cCadC-NtRbcL (rbcL Synthetic)

Expression

Bacterial

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pSL012

Plasmid

#246580

Purpose

express mGreen Lantern from the cadBA promoter

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

mGreenLantern

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTW368z

Plasmid

#246581

Purpose

Golden gate acceptor for AtRaf1 library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

sfGFP, AtRaf2, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (RbcX2, CPN60B, CPN20, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSYL018

Plasmid

#246594

Purpose

template plasmid for site-saturation mutagenesis library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1 variant 2b (AT3G04550 Mustard Weed)

Expression

Bacterial

Mutation

AtRaf1I90V/I127F/R139H/M243K/E275V/V334A/K411I/R4…

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBL155

Plasmid

#246591

Purpose

express cCadC AtRbcL fusion protein

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

cCadC-AtRbcL (rbcL Synthetic)

Expression

Bacterial

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e3

Plasmid

#246588

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, CPN60B, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Nov. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411b

Plasmid

#246583

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 )

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSYL017

Plasmid

#246593

Purpose

template plasmid for site-saturation mutagenesis library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1 variant 2g (AT3G04550 Mustard Weed)

Expression

Bacterial

Mutation

AtRaf1L121F/I127L/A195V/N351Y/R432G

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e2b

Plasmid

#246587

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtRbcX2, AtBSD2W108A/L109E, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)

Expression

Bacterial

Mutation

AtBSD2W108A/L109E

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e4

Plasmid

#246589

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, RbcX2, CPN60B, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e2

Plasmid

#246586

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtRbcX2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411c

Plasmid

#246584

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411a

Plasmid

#246582

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN20, CPN60A, AT3G04550 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSIn-H3K9ac Y97F BS

Plasmid

#235132

Purpose

Mutant (negative control) Y97F biosensor for pSin-H3K9ac Full

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1Y97F-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Mutation

BD1Y97F

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-Strep-KDEL

Plasmid

#228527

Purpose

Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

Strep-KDEL

Use

Lentiviral

Tags

Streptavidin-KDEL

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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p4P1R_EC1.2en-EC1.1p

Plasmid

#213912

Purpose

Entry clone containing Egg cell-specific promoter (EC1.1p) fused to EC1.2 enhancer (EC1.2en). For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

Insert

EC1.2en-EC1.1p

Expression

Plant

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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