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pAS1-lim-4P::his-24::Cerulean

Plasmid

#124336

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

lim-4, Cerulean (lim-4 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BFP-ecDHFR-NES-SV40polyA

Plasmid

#252089

Purpose

Expresses BFP-ecDHFR-NES in mammalian cells

Depositor

Karl Deisseroth

Publication

Zhao et al Nat Chem. 2026 Jul 8. doi: 10.1038/s4155

Insert

BFP-ecDHFR-NES

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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MBP-Streptavidin-RGG

Plasmid

#242451

Purpose

Expresses the MBP-Streptavidin-RGG fusion protein in E Coli.

Depositor

Benjamin Schuster

Publication

Kelley et al Nat Commun. 2025 Apr 14;16(1):3521. doi:

Insert

MBP-Streptavidin-RGG (using LAF-1 RGG domain)

Tags

MBP and Steptavidin

Expression

Bacterial

Available since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Publication

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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KVS1-HA

Plasmid

#113061

Purpose

Expresses worm KVS-1 in mammalian cells

Depositor

Federico Sesti

Publication

Cai et al Nat Neurosci. 2005 Nov;8(11):1503-9. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)

Tags

HA

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEG459

Plasmid

#40088

Depositor

Geraldine Seydoux

Publication

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S404A) (mex-5 Nematode)

Tags

MBP

Expression

Bacterial

Mutation

S404A

Promoter

P-lac

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pDMG8a

Plasmid

#33179

Depositor

David Bartel

Publication

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

C27H6.9 3'UTR (C27H6.9 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG1a

Plasmid

#33158

Depositor

David Bartel

Publication

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

cog-1 3'UTR (cog-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDP-let-7_LPB

Plasmid

#26560

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPB

Use

Transcription vector

Mutation

loop mutant B

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNAOX-twk46

Plasmid

#16129

Depositor

Larry Salkoff

Publication

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Voltage-gated K+ channel (twk-46 Nematode)

Use

Mammalian expression & oocyte expression

Mutation

N/A

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pHDR2_mGreenLantern-T2A-SYCP3

Plasmid

#222523

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to SYCP3.

Depositor

George Church

Publication

Pierson Smela et al bioRxiv 2024.05.31.596483

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mGreenLantern

Use

CRISPR

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SL2 GFP H2B

Plasmid

#227717

Purpose

Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Publication

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SL2 GFP H2B (nlp-51 Nematode)

Use

Cloning vector

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

stop at beginning of last exon

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mg172

Plasmid

#34652

Depositor

Miriam Goodman

Publication

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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mg182

Plasmid

#34662

Depositor

Miriam Goodman

Publication

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B2;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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U653-GFP ILtoEK

Plasmid

#24473

Depositor

Ron Vale

Publication

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

I362E/L365K (neck destabilization mutant)

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pJL2

Plasmid

#184130

Purpose

For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

Depositor

Alejandro Aballay

Publication

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Publication

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only