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Flag pLJM1 RagB 54L

Plasmid

#19314

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T54L

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-TagRFP-t35s

Plasmid

#118386

Purpose

Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p-TagRFP

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

Plasmid

#179003

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC 75L

Plasmid

#19305

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Tags

GST and HA

Expression

Mammalian

Mutation

S75L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Article

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Insert

Nav1.2 (Scn2a Rat)

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Vimentin/pcDNA3

Plasmid

#67772

Purpose

Vimentin-targeted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor-Vimentin

Tags

Vimentin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pH-tdGFP

Plasmid

#74322

Purpose

pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

Depositor

Joerg Stelling

Article

Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

Insert

pH-tdGFP

Use

Yeast tagging

Tags

none

Expression

Yeast

Mutation

N149Y, Q204H

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB wt

Plasmid

#19301

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA wt

Plasmid

#19298

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pBad33_ISTfu1_reRNA

Plasmid

#205355

Purpose

Bacterial expression of ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISTfu1 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pECFP-Sec31A

Plasmid

#66612

Purpose

CFP fusion of Sec31A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec31A (SEC31A Human)

Tags

ECFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/p-bacteria

Plasmid

#64325

Purpose

Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

Insert

3xFLAG-dCas9

Use

CRISPR; Tetracycline-inducible

Tags

3xFLAG

Expression

Bacterial

Mutation

D10A, H840A

Promoter

pLtetO-1

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U1-70K-fSNAPc

Plasmid

#106987

Purpose

open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

U1-70K (SNRNP70 Human)

Tags

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-eyfp-a

Plasmid

#111620

Purpose

eYFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-eyfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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Map3k1

Plasmid

#29581

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Map3k1 (MAP3K1 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively Active. Contains catalytic domain …

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCDB179-BhrPETase

Plasmid

#218692

Purpose

Encodes codon optimized BhrPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

BhrPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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SPDK3959

Plasmid

#199246

Purpose

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

Insert

Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Promoter

PEBV

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pFUGW-hGtACR1-EYFP

Plasmid

#67795

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Article

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR1

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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