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Tol2-LyzC-RacFRET-U6ac-ctrl guides

Plasmid

#168247

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-RacFRET-U6ac-rac2 guides

Plasmid

#168248

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-zdgat2FLAG-SV40

Plasmid

#232141

Purpose

Expresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Zebrafish expression

Tags

3xFLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

Plasmid

#168251

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

Plasmid

#168254

Purpose

"label stable F-actin under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

Plasmid

#168252

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

Plasmid

#168253

Purpose

"label stable F-actin; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt300 2n-KalNFB-syt2a

Plasmid

#196901

Purpose

zebrafish expression

Depositor

Reinhard Köster

Insert

nbt300 2n-KalNFB-syt2a

Use

Zebrafish expression

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-ZSP:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194518

Purpose

ZSP promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+-eGFP

Plasmid

#221710

Purpose

Expression of eGFP in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

eGFP

Use

Zebrafish expression

Promoter

CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#193851

Purpose

E1b promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-fos:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194516

Purpose

fos promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194515

Purpose

P2 promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-P0.8:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194514

Purpose

P0.8 promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-slc1a3b

Plasmid

#170205

Purpose

Gateway 5’ entry vector with the slc1a3b promoter

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b )

Use

Unspecified; Zebrafish

Promoter

slc1a3b

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f

Plasmid

#209283

Purpose

Expresses Voltron1 and jGCaMP7f in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

Voltron1_P2A_jGCaMP7f

Use

Zebrafish expression

Promoter

zACNE20_myl7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pISceI Dusp6:D2EGFP

Plasmid

#32667

Depositor

Michael Tsang

Article

Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pTol2-rtn1a-KALTA4

Plasmid

#239989

Purpose

Expresses GAL4 under control of the rtn1a promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and rtn1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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