Addgene

We narrowed to 4,844 results for: ATS

Showing: 321 - 340 of 4844 results

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LentiCRISPRv2-neo-dTgfa

Plasmid

#173842

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-dEreg

Plasmid

#173843

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-puro-dEgfr

Plasmid

#173844

Purpose

A knockout vector for the dog Egfr.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisHbegf

Plasmid

#173846

Purpose

Encoding dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Hbegf.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisTgfa

Plasmid

#173847

Purpose

Encoding dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEreg

Plasmid

#173848

Purpose

Encoding dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Raichu-454HRasCT

Plasmid

#176029

Purpose

A FRET biosensor for HRas activity

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

Raichu-Ras-HRasCT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139485

Purpose

PCR template for Halo-Tag gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

HaloTag-loxP-P2A-dTKneo-pA-loxP

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1

Plasmid

#17715

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

pRS306Sup35R1 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

An pRS306 based integrating plasmid designed for …

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-loxP-canisNrg1

Plasmid

#173849

Purpose

Encoding dog Nrg1 before Cre-mediated recombination.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Nrg1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEgf

Plasmid

#173845

Purpose

Encoding dog Egf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis EGF.

Use

Tags

Expression

Mammalian

Mutation

R1127Q- please see dep. comments

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-MVP

Plasmid

#204543

Purpose

Expresses EGFP-tagged MVP in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

Plasmid

#209922

Purpose

A knock-out vector for the dog CDH1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CDH1 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

Plasmid

#209923

Purpose

A knock-out vector for the dog CTNND1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CTNND1 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-mNeonGreen

Plasmid

#209913

Purpose

To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-mNeonGreen (TGFA Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IRES-puro-3xFLAG

Plasmid

#204545

Purpose

Expresses 3xFLAG tag in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

3xFlag

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP

Plasmid

#204541

Purpose

Expresses GST-tagged MVP in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Plasmid

#186703

Purpose

Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IRES-puro-3xFLAG-NBR1

Plasmid

#204546

Purpose

Expresses 3xFLAG-tagged NBR1 in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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