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pX459-dErbB2

Plasmid

#197355

Purpose

A knock-out vector for dog ErbB2.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_1

Plasmid

#190703

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DPA

Plasmid

#164988

Purpose

Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPA1*02:01:08

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.1 (Hist1h3g) sgRNA

Plasmid

#186930

Purpose

Hist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hist1h3g sgRNA (H3c8 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRAF

Plasmid

#183273

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRAF gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRAF

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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1056F

Plasmid

#183134

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[tra]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ROS1

Plasmid

#183320

Purpose

All-in-One CRISPRko system with a guide RNA that targets ROS1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ROS1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 smFP-HA-Cacna1e KI

Plasmid

#183441

Purpose

FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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gh41

Plasmid

#106715

Purpose

expression of gRNA targeting ST6GAL2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GAL2 (ST6GAL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1323

Plasmid

#111125

Purpose

Expresses non-targeting_01224 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

Non-targeting gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF644_2

Plasmid

#86306

Purpose

Encodes gRNA for 3' target of human ZNF644

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF644 (ZNF644 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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shCDK4(1)

Plasmid

#73550

Purpose

shRNA(1) against CDK4

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-A5T

Plasmid

#62264

Purpose

expression of A5T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5NT

Plasmid

#62263

Purpose

expression of A5NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4T

Plasmid

#62262

Purpose

expression of A4T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A1NT

Plasmid

#62255

Purpose

expression of A1NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1NT

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Lmo3.8 shRNA

Plasmid

#59294

Purpose

shRNA to mouse Lmo3

Depositor

Ulrike Heberlein

Article

Lasek et al Alcohol Clin Exp Res. 2011 Sep;35(9):160

Insert

Lmo3.8 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pBS-Histone H3 imperfect loop

Plasmid

#45087

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2014 Jul;141(13):2592-601.

Insert

Histone H3 imperfect stem

Use

RNAi; In vitro transcription

Tags

Expression

Mutation

stem loop sequence is naturally occurring mutant …

Promoter

T7 sense, T3 antisense

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only
QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

TaU3

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

Plasmid

#70658

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only