We narrowed to 2,323 results for: PUC19

1. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

   Plasmid

   #195505

   Purpose

   T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
   

   Use

   CRISPR; Donor vector

   Tags

   mRuby-3xFLAG-NLS-3xSV40-poly(A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

   Plasmid

   #121175

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFloxed DHFR-TS*

   Plasmid

   #100605

   Purpose

   pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR

   Depositor

   David Sibley

   Insert

   Floxed TgDHFR-TS*
   

   Use

   Toxoplasma expression

   Tags

   Expression

   Mutation

   Pyrimethamine resistance

   Promoter

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-ODN

   Plasmid

   #208642

   Purpose

   Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Long single strand dna production

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR

   Plasmid

   #113252

   Purpose

   pUC19 backbone with E. coli CRISPR array

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   E. coli CRISPR array
   

   Use

   Integration substrate

   Tags

   Expression

   Mutation

   Promoter

   NA

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW091

   Plasmid

   #113253

   Purpose

   pUC19 backbone with S. pyogenes CRISPR array

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes CRISPR array
   

   Use

   Integration substrate

   Tags

   Expression

   Mutation

   Promoter

   NA

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-T7-J1~398-Luc

   Plasmid

   #208639

   Purpose

   for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19

   Depositor

   Quanfu Ma

   Insert

   Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
   

   Use

   Luciferase; In vitro transcription template

   Tags

   Firefly Luciferase (F-Luc and T7 promoter

   Expression

   Mutation

   nt 1~398, in frame with donstream F-Luc

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT440

   Plasmid

   #8609

   Depositor

   Robert Hartley

   Article

   Yazynin et al Gene 1996 Feb 22;169(1):131-2.

   Insert

   barnase, barstar
   

   Use

   Tags

   as pMT416

   Expression

   Bacterial

   Mutation

   V36 codon of barnase of pMT416 replaced by polyli…

   Promoter

   Available since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pSelAct-KO

    Plasmid

    #174374

    Purpose

    Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

    Depositor

    Gitta Coaker

    Article

    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

    Type

    Empty backbone

    Use

    Vector for 2-step knockout by homologous recombin…

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSelAct-Express

    Plasmid

    #174375

    Purpose

    Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible

    Depositor

    Gitta Coaker

    Article

    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

    Type

    Empty backbone

    Use

    Expression vector for targeted integration

    Tags

    Expression

    Mutation

    Promoter

    pCMP1

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHB1

    Plasmid

    #204668

    Purpose

    pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

    Depositor

    Mark Mandel

    Article

    Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

    Insert

    FRT-erm-FRT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCVD432

    Plasmid

    #63755

    Purpose

    Probe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.

    Depositor

    James Kaper

    Article

    Baudry et al J Infect Dis. 1990 Jun;161(6):1249-51.

    Insert

    EAggEC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pILGFP1DFB

    Plasmid

    #185871

    Purpose

    A parental plasmid for in vivo gene amplification at RPL25 locus

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-T7-hTP53-P0~670-FLuc

    Plasmid

    #208640

    Purpose

    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.

    Depositor

    Quanfu Ma

    Insert

    TP53, UTR and partial CDS (TP53 Human)
    

    Use

    Luciferase; In vitor transcription template

    Tags

    Firefly Luciferase (F-Luc) and T7 promoter

    Expression

    Mutation

    UTR and partial CDS in-frame with F-Luc

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT1004

    Plasmid

    #8613

    Depositor

    Robert Hartley

    Insert

    binase, barstar
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    as pMT1002

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pILGFP4BG6

    Plasmid

    #185877

    Purpose

    A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pILGFP3AA5

    Plasmid

    #185875

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pILGFP3AG4

    Plasmid

    #185874

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge