We narrowed to 1,121 results for: gibson assembly

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pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Plasmid

#137110

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-MMP

Plasmid

#112632

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-MMP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central matrix metalloproteinase-cleavable sequen…

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p005-RFP-weak

Plasmid

#108316

Purpose

Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of weak synthetic constitutive promoter pFAB4024

Expression

Bacterial

Promoter

pFAB4024

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MZB117

Plasmid

#228481

Purpose

"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)

Depositor

Richard Baker

Type

Empty backbone

Tags

GST (glutathione S-transferase)

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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P18m

Plasmid

#120941

Purpose

MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

T7

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C31m

Plasmid

#120966

Purpose

MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

bxb1 integrase

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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P9m

Plasmid

#120937

Purpose

MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file

Depositor

Richard Murray

Insert

Pr-OR1-OR2

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MZB186

Plasmid

#228489

Purpose

Empty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

Depositor

Richard Baker

Type

Empty backbone

Tags

10xHis-SUMO(Smt3)-Avitag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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U9m

Plasmid

#121022

Purpose

MoClo golden gate assembly BC part for BCD22 (very low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

Depositor

Richard Murray

Insert

BCD22

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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T1m

Plasmid

#121028

Purpose

MoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).

Depositor

Richard Murray

Insert

T500_noGap, short attachment (T11)

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C113m

Plasmid

#121012

Purpose

MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

Cas9

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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U2m

Plasmid

#121015

Purpose

MoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

Depositor

Richard Murray

Insert

BCD11

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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U3m

Plasmid

#121016

Purpose

MoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

Depositor

Richard Murray

Insert

BCD13

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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U5m

Plasmid

#121018

Purpose

MoClo golden gate assembly BC part for BCD14 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

Depositor

Richard Murray

Insert

BCD14

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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U6m

Plasmid

#121019

Purpose

MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

Depositor

Richard Murray

Insert

BCD20

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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U7m

Plasmid

#121020

Purpose

MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

Depositor

Richard Murray

Insert

BCD16

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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U8m

Plasmid

#121021

Purpose

MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

Depositor

Richard Murray

Insert

BCD24

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C84m

Plasmid

#120995

Purpose

MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

Sigma 28

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C95m

Plasmid

#121001

Purpose

MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

T7 RNA polymerase (Andy please check!)

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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C63m

Plasmid

#120979

Purpose

MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

lacI

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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