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pBW701_pCAG-PV1

Plasmid

#87561

Purpose

Simple PV1

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW704_pCAG-PV4

Plasmid

#87564

Purpose

Simple PV4

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW703_pCAG-PV3

Plasmid

#87563

Purpose

Simple PV3

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET23MutS-F39A

Plasmid

#13044

Depositor

Peggy Hsieh

Article

Malkov et al J Biol Chem. 1997 Sep 19. 272(38):23811-

Insert

MutS

Expression

Bacterial

Mutation

Phe39Ala mutation Full length

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCU307D

Plasmid

#243806

Purpose

A small helper Ti-plasmid

Depositor

Hiroharu Banno

Insert

vir region genes

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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NSK242 SFFV-mCherry-SGc(os13.NOT-SMN2.36,55,36)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247283

Purpose

modulADAR sensor for NOT SMN exon 7

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-os13.NOT-SMN2.36,55,36-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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NSK267 SFFV-mCherry-SGc(EBER2.linear.1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247288

Purpose

Linear sensor for EBER2

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-os13.EBER2.linear.1-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK266 SFFV-mCherry-SGc(EBER1.linear.2)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247287

Purpose

Linear sensor for EBER1

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-os13.EBER1.linear.2-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK173 SFFV-mCherry-SGc(Smn2ex7.os13-72bp)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247277

Purpose

modulADAR sensor for SMN exon 7

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-Smn2ex7.os13-72bp)-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EK0656 SFFV-mCherry-SGc(70587.os4)cSG-EGFP-(3xMS2)

Plasmid

#247269

Purpose

modulADAR sensor against sythetic target transcript (syn GluR-B stemloop)

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-s70587.os4-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK241 SFFV-mCherry-SGc(os13.NOT-SMN2.45,55,45)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247282

Purpose

modulADAR sensor for NOT SMN exon 7

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-os13.NOT-SMN2.45,55,45-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-32-mCherry

Plasmid

#234062

Purpose

Encodes a Melt variant with a switching temperature of 32°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-32-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGB010

Plasmid

#223678

Purpose

pEF-HA-NLS-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGH1327

Plasmid

#223679

Purpose

pEF-HA-PYL1-NLSminus-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSL187

Plasmid

#223680

Purpose

pEF-HA-3xNES-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

Plasmid

#222861

Purpose

This plasmid codes for the OgeuIscB nickase

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB Nickase

Use

CRISPR

Tags

3X HA and Nucleoplasmin NLS and ITR2

Expression

Mammalian

Mutation

E193A for nickase mutation

Promoter

CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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XLone-BSD NFIL3 T2A Puro

Plasmid

#140028

Purpose

Tunable and temporal expression control of NFIL3 and puro resistant gene

Depositor

Xiaoping Bao

Insert

NFIL3 (NFIL3 Human, Synthetic)

Use

Synthetic Biology

Tags

puromycin

Expression

Mammalian

Promoter

TRE3G

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-ILVBLpA-G10C

Plasmid

#120206

Purpose

Human ILVBL poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-del

Plasmid

#120200

Purpose

human RPS5 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2r

Plasmid

#120199

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2

Plasmid

#120198

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m1r

Plasmid

#120197

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m1

Plasmid

#120196

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA-del

Plasmid

#120193

Purpose

human CENPB poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA-del

Plasmid

#120204

Purpose

human TFE3 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA-G9C

Plasmid

#120202

Purpose

Human TFE3 poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pStrep-5xEGFP-N1

Plasmid

#122492

Purpose

Expression of Strep-tagged 5xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 5xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pStrep-7xEGFP-N1

Plasmid

#122494

Purpose

Expression of Strep-tagged 7xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 7xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pStrep-8xEGFP-N1

Plasmid

#122495

Purpose

Expression of Strep-tagged 8xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 8xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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