We narrowed to 8,836 results for: FIE

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ARB1

Plasmid

#232888

Purpose

Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ARB1 gRNA (ARB1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-RER2

Plasmid

#232884

Purpose

Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

RER2 gRNA (RER2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-3

Plasmid

#232894

Purpose

Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LEU2 gRNA (RER2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS313_MSN5-LY00018

Plasmid

#232903

Purpose

Plasmid carrying MSN5 from LY00018 (YJM975) with its native promoter and terminator.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

MSN5 (MSN5 Budding Yeast)

Expression

Yeast

Mutation

G1563A

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4-URA3-3

Plasmid

#232886

Purpose

Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

URA3 gRNA (URA3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4-URA3-2

Plasmid

#232885

Purpose

Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

URA3 gRNA (URA3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-AVO1

Plasmid

#232889

Purpose

Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

AVO1 gRNA (AVO1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-3

Plasmid

#232882

Purpose

Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-YFR054C

Plasmid

#232900

Purpose

Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

YFR054C gRNA (YFR054C Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ZIM17

Plasmid

#232901

Purpose

Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ZIM17 gRNA (ZIM17 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SRP14

Plasmid

#232896

Purpose

Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SRP14 gRNA (SRP14 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-TLG1

Plasmid

#232897

Purpose

Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

TLG1 gRNA (TLG1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-VHT1

Plasmid

#232898

Purpose

Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VHT1 gRNA (VHT1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-2

Plasmid

#232881

Purpose

Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ADE13

Plasmid

#232887

Purpose

Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ADE13 gRNA (ADE13 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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