We narrowed to 5,185 results for: PID;

1. MTK3_018

   Plasmid

   #123733

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   spdCas9(1-204)-frb-D10A-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY71-tet.I.4

   Plasmid

   #133526

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY71-tet.I.1

   Plasmid

   #133523

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOVC1_X_AsPT1-LOV2-EcSsra

   Plasmid

   #131217

   Purpose

   Expresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   AsPT1-LOV2-EcSsra
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOVC1_X_AsPT1-LOV2-pep

   Plasmid

   #131215

   Purpose

   Expresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   AsPT1-LOV2-pep
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMH-HT_CtCHAD-R438A-K441A

   Plasmid

   #127791

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in Arg4…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-SAC1C389S-HLx8

   Plasmid

   #108140

   Purpose

   Inactive SAC1 with extended linker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pK RagA(R37P)-RagC

   Plasmid

   #99706

   Purpose

   Bacterial expression of RagA(R37P)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   R37P

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK RagA-RagC(L91P)

   Plasmid

   #99707

   Purpose

   Bacterial expression of RagA-RagC(L91P)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   L91P

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pK RagA-RagC(K84T)

    Plasmid

    #99708

    Purpose

    Bacterial expression of RagA-RagC(K84T)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    K84T

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pK RagA-RagC(S75N)

    Plasmid

    #99699

    Purpose

    Bacterial expression of RagA-RagC(S75N)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    S75N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETDuet RagA(R37P)-RagC

    Plasmid

    #99646

    Purpose

    Bacterial expression of RagA(R37P)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    R37P

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETDuet RagA-RagC(K84T))

    Plasmid

    #99648

    Purpose

    Bacterial expression of RagA-RagC(K84T)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    K84T

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pK RagA(T21N)-RagC

    Plasmid

    #99650

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    T21N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMDC84-ScSEIPIN-Ndelta

    Plasmid

    #96985

    Purpose

    Express yeast SEIPIN gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    ScSEIPIN (SEI1 Budding Yeast)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    N-terminal 39 nucleotides were deleted from yeast…

    Promoter

    35S

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS315-PGK-SEIPIN2

    Plasmid

    #96987

    Purpose

    Express GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN2 (AT1G29760 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Phosphoglycerate Kinase (PGK) promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMDC84-SEIPIN3N-1C

    Plasmid

    #96983

    Purpose

    Express mutated Arabidopsis SEIPIN1 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN1 (AT5G16460 Mustard Weed)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    A segment of 507 nucleotides from the N terminus…

    Promoter

    35S

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMDC84-SEIPIN1N-3C

    Plasmid

    #96982

    Purpose

    Express mutated Arabidopsis SEIPIN3 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN3 (AT2G34380 Mustard Weed)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    A segment of 108 nucleotides from the N terminus…

    Promoter

    35S

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

    Plasmid

    #91935

    Purpose

    Bacterial expression of maize SUMO1a and inactive SCE1d

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1d(C94S)-Myc
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    C94S active site mutation and Truncation of ZmSUM…

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

    Plasmid

    #91933

    Purpose

    Bacterial expression of maize SUMO1a and inactive SCE1b

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1b(C94S)-Myc
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    C94S active site mutation and Truncation of ZmSUM…

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge