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pMyBADNT

Plasmid

#84690

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

AraC

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-ApplePy1Highest

Plasmid

#239064

Purpose

Mammalian expression of highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Highest

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGoldenGate-SE9

Plasmid

#47677

Purpose

Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.

Depositor

Jose Dinneny

Article

Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

Type

Empty backbone

Use

Arabidopsis transformation

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pMyC

Plasmid

#42192

Depositor

Annabel Parret, Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

ColE1

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-GreenPy1Highest

Plasmid

#239059

Purpose

Mammalian expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

GreenPy1Highest

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMi(3xP3-DsRed)

Plasmid

#102539

Purpose

Transgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed marker

Depositor

Michalis Averof

Article

Pavlopoulos et al Proc Natl Acad Sci U S A. 2005 May 31;10

Insert

Minos transpoable element with 3xP3-DsRed marker

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pB-actin E6 E7

Plasmid

#13712

Depositor

Karl Munger

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV 16 E6 E7 (E6, E7 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-GreenPy1Low

Plasmid

#239061

Purpose

Mammalian expression of low affinity green fluorescent pyruvate biosensor GreenPy1Low

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

GreenPy1Low

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-dGreenPy1

Plasmid

#239073

Purpose

Mammalian expression of mitochondria-targeted green fluorescent pyruvate control biosensor dGreenPy1

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

dGreenPy1

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-ApplePy1Low

Plasmid

#239066

Purpose

Mammalian expression of low affinity red fluorescent pyruvate biosensor ApplePy1Low

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Low

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCLAP

Plasmid

#200786

Purpose

Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Type

Empty backbone

Tags

CLIPf and SNAPf-6xHis-FLAG

Expression

Mammalian

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PKC epsilon DN

Plasmid

#21243

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC epsilon (Prkce Mouse)

Tags

HA

Expression

Mammalian

Mutation

K437R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pET21a asyn(Δ41-54)

Plasmid

#242234

Purpose

Expresses alpha synuclein (missing exon 3, residues 41-54) in bacteria

Depositor

Jennifer Lee

Article

SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

Insert

asyn(Δ41-54)

Tags

none

Expression

Bacterial

Mutation

deleted amino acids 41-54

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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PKC eta DN

Plasmid

#21246

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Mutation

K384R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
p6-8

Plasmid

#118089

Purpose

rapa-T7- mRNA(GFP)

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

Rapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP

Expression

Mammalian

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAd5-B6 ∆E3-GFP

Plasmid

#179201

Purpose

Block 6 with deletion in E3 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6, with deletion in E3 and insertion of GFP expression cassette

Use

Adenoviral

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p5-79

Plasmid

#118087

Purpose

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Use

Synthetic Biology

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe GFP Small T antigen

Plasmid

#10673

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
SpPLK1-GFP

Plasmid

#185573

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-Myc-MBP-HA

Plasmid

#222711

Purpose

Expression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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