We narrowed to 57,378 results for: plasmid
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pGBW-m4046852
Plasmid#145584PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAST_mLExA-VP16-NFATc1 (C-del)
Plasmid#35023DepositorInsertNFATc1 (NFATC1 Human)
TagsVP16 and mLexAExpressionInsectMutationdeletion of amino acids 590-716PromoterUASAvailable SinceMarch 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mArg1-Flag
Plasmid#34574DepositorAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-dTAG-Puro
Plasmid#207727PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4.10/RSV_SF3B3-Xon_Luciferase
Plasmid#174659PurposePlasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.DepositorInsertXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG2a_M18
Plasmid#105930PurposeExpression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.DepositorAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57 (Gent)
Plasmid#54338DepositorTypeEmpty backboneUseCloning vectorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike-d18
Plasmid#164583PurposeA mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.DepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
ExpressionMammalianMutationDeleted amino acids 1256-1273PromoterEF1aAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)-IgG1
Plasmid#154104PurposeMammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti.Cas9.BFP.Blast
Plasmid#196714PurposeWT SpCas9 with BSD-TagBFP for selection and sortingDepositorInsertCas9-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
TagsHexahistidine tag with Thrombin cleavageExpressionBacterialPromoterT7Available SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-64: FUS-mEGFP
Plasmid#114410PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human FUSDepositorInsertFUS Homology Arms with linker-mEGFP (FUS Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceSept. 5, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable SinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIB166
Plasmid#90189PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-B19M
Plasmid#59923PurposeExpression vector for Rabies virus SAD B19 matrix protein geneDepositorInsertRabies virus matrix protein
ExpressionMammalianPromoterCAGAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Amp
Plasmid#92051PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRPromoterpRhamBAD and ptetAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-135
Plasmid#118553PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-60: SOX2-mEGFP
Plasmid#124606PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2DepositorInsertSOX2 Homology Arms with linker-mEGFP (SOX2 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceMay 13, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits
