We narrowed to 20,261 results for: ACE;

1. pGL3-Basic-hCYP26A1P-E4-mCherry

   Plasmid

   #135478

   Purpose

   Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   mCherry Open reading Frame
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Short from promoter of human CYP26A1 (312 bp)

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTarget TMEM97-P2A-mCardinal

   Plasmid

   #157747

   Purpose

   Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

   Depositor

   Andrew Kruse

   Article

   Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

   Insert

   TMEM97 (TMEM97 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. MTK2_001

   Plasmid

   #123697

   Purpose

   Encodes pTRE as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pTRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK510

   Plasmid

   #110611

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. RnBarr1-mVenus

   Plasmid

   #137790

   Purpose

   Visualization of beta-arrestin1

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin 1 (Arrb1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209655

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Has Service

   AAV5

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

   Plasmid

   #154829

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   coEPO
   coEPO
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized (Cricetulus griseus)

   Promoter

   100RPU.2

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-Basic-hCYP26A1-FL- luciferase

   Plasmid

   #135566

   Purpose

   Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

   Insert

   Full-length promoter of human retinoic acid receptor-beta gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   hHuman CYP26A1 gene promoter

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HiFi dCas9 KRAB

   Plasmid

   #188500

   Purpose

   Expresses FLAG tagged HiFi dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/R691A/H840A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. KBB364

    Plasmid

    #185083

    Purpose

    RFA1 fused to GFP under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEasyG1_hph

    Plasmid

    #184912

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLP-mScarlet-I

    Plasmid

    #209993

    Purpose

    Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK599

    Plasmid

    #110600

    Purpose

    BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP dropout
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTU1-A-SP44-mVenus-I

    Plasmid

    #163763

    Purpose

    Expresses Streptomyces codon-optimised mVenus-I

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    mVenus-I
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    SP44

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. plko.1/shMCT4 #474

    Plasmid

    #99597

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPReporter

    Plasmid

    #65007

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Stra6L-6His

    Plasmid

    #174215

    Purpose

    Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

    Depositor

    Adrian Press

    Article

    Stafast et al ACS Appl. Polym. Mater. 2022

    Insert

    Stra6L (Stra6l Mouse)
    

    Use

    Tags

    6-His

    Expression

    Mammalian

    Mutation

    non

    Promoter

    CMV

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Fluorescent reporter for MOBE3-4

    Plasmid

    #219946

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAH-CTX1-rhadCas9-native

    Plasmid

    #129392

    Purpose

    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine, Histidine 840 to Alanine

    Promoter

    PrhaBAD

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTU2-A-RFP (colE1 origin)

    Plasmid

    #74093

    Purpose

    pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    R0010 Const. Promoter controlling RFP

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. GW1-mito-mCherryEA

    Plasmid

    #207868

    Purpose

    Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

    Depositor

    Mathew Tantama

    Article

    Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

    Insert

    mito-mCherryEA
    

    Use

    Tags

    4xCox8 mitochondrial targeting

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBTK527a

    Plasmid

    #110606

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. HA-MBP-TEVpCS-ADGRL1 CTF-FLAG

    Plasmid

    #217706

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL1 (ADGRL1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-838

    Promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTU2S-b (p15A origin)

    Plasmid

    #74089

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

    Plasmid

    #22930

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaPH (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes 127-1205 aa. Deleted amino-terminal PH d…

    Promoter

    CMV

    Available Since

    Feb. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pBTK509

    Plasmid

    #110610

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

    Plasmid

    #118000

    Purpose

    Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    Derlin1-mOrange2
    

    Use

    Plant/bacteria binary vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin-10

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBTK121

    Plasmid

    #110581

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    PA3 Promoter with RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. CaP2AIII_pK18

    Plasmid

    #153421

    Purpose

    Source of murine TCR-alpha constant region (TRAC*02) gene followed by P2A

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha constant region (TRAC*02)-P2A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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