We narrowed to 145,110 results for: ins

1. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-X1-Neo-GFP-FAM134B-LIRmut

   Plasmid

   #109027

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   FAM134B-LIRmut (RETREG1 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

   Promoter

   EIF-1a promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 3xFlag IFIT1

   Plasmid

   #53554

   Purpose

   mammalian expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

   Plasmid

   #185713

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed W403 and F753 to A

   Promoter

   T7

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRII_TOPO_hNEAT1

   Plasmid

   #61518

   Purpose

   human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

   Depositor

   Archa Fox

   Article

   Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

   Insert

   NEAT1 (NEAT1 Human)
   

   Use

   General cloning vector

   Tags

   Expression

   Mutation

   *see comment below

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CapChR2_pmScarlet-N1

   Plasmid

   #188032

   Purpose

   Expresses CapChR2-mScarlet in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMW363

   Plasmid

   #238208

   Purpose

   Expresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Use

   Tags

   TwinStrepII

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pM-mClover3

    Plasmid

    #228624

    Purpose

    Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL7.0: tgRFPt-SSPB WT

    Plasmid

    #60415

    Purpose

    Mammalian Expression of tgRFPt-Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    tgRFPt-SSPB
    

    Use

    Cre/Lox and Lentiviral

    Tags

    TagRFPt

    Expression

    Mammalian

    Mutation

    Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUASTattB-dALFANb(5mut)-mGreenLantern

    Plasmid

    #219661

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-HcKCR1-EYFP

    Plasmid

    #182021

    Purpose

    Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

    Depositor

    Mingshan Xue

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Venus-1-155-Ggamma2

    Plasmid

    #234752

    Purpose

    Mammalian expression of BiFC Venus-betagamma (with Venus-155-239-Gbeta1)

    Depositor

    Nevin Lambert

    Article

    Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

    Insert

    GNG2 (GNG2 Human)
    

    Use

    Tags

    VC155

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus-155-239-Gbeta1

    Plasmid

    #234751

    Purpose

    Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

    Depositor

    Nevin Lambert

    Article

    Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

    Insert

    GNB1 (GNB1 Human)
    

    Use

    Tags

    VN155

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Use

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL7.0: Venus-iLID-Mito (From ActA)

    Plasmid

    #60413

    Purpose

    Mammalian Expression of Venus-iLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. hGip-R

    Plasmid

    #14942

    Depositor

    Bernard Thorens

    Article

    Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

    Insert

    Gip-R (GIPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Short form of the receptor

    Promoter

    Available since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

19. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Use

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-RT004-LaG30VB

    Plasmid

    #163384

    Purpose

    2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG30-VEEGFRtm-BFP (LaG30VB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLL7.0: tgRFPt-SSPB R73Q

    Plasmid

    #60416

    Purpose

    Mammalian Expression of tgRFPt-Micro

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    tgRFPt-SSPB R73Q
    

    Use

    Cre/Lox and Lentiviral

    Tags

    TagRFPt

    Expression

    Mammalian

    Mutation

    R73Q, Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBABE puro human IRS-1

    Plasmid

    #11025

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMIIS793

    Plasmid

    #238206

    Purpose

    Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    TwinStrepII-bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLL7.0: Venus-iLID-CAAX (from KRas4B)

    Plasmid

    #60411

    Purpose

    Mammalian Expression of Venus-iLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p10xQUAS-CsChrimson

    Plasmid

    #163629

    Purpose

    10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

    Depositor

    Christopher Potter

    Article

    Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

    Insert

    CsChrimson.mVenus
    

    Use

    Tags

    mVenus

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pACE-GIRK4-mC-S

    Plasmid

    #172427

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    mCherry Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pR I-Ab alpha BirA

    Plasmid

    #113638

    Purpose

    Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    I-Ab alpha BirA (H2-Ab1 Mouse)
    

    Use

    Tags

    BirA biotinylation signal site and fused to Fos-J…

    Expression

    Insect

    Mutation

    transmembrane and cytoplasmic domains truncated

    Promoter

    Metallothionein

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pQE-80L MBP-SspB Nano

    Plasmid

    #60409

    Purpose

    E. coli expression of SspB Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    SspB nano
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Y11K and A15E

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-mNeptune2-N

    Plasmid

    #41645

    Purpose

    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    mNeptune2-Dronpa145N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K145N on Dronpa (allows control of photochromic F…

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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30. pJRoC30_VP8H

    Plasmid

    #188062

    Purpose

    PROSS-designed versatile peroxidase 8H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    

    Use

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    38 PROSS mutations, described in publication

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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