We narrowed to 4,086 results for: PRS

1. csr-1_son-1_mNeonGreen

   Plasmid

   #191743

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mNeonGreen

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MK1283

   Plasmid

   #71428

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Use

   Tags

   Expression

   Mutation

   T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

   Promoter

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-X0

   Plasmid

   #115565

   Purpose

   Control vector expressing GFP alone without any consensus repeats

   Depositor

   Michael Downey

   Article

   Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

   Insert

   Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYZ959

   Plasmid

   #192603

   Purpose

   TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   ScTEF1

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. bRA77

   Plasmid

   #100953

   Purpose

   GAL1 driven Cas9. gRNA can be cloned into BplI sites.

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pG3

   Plasmid

   #162604

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYZ960

   Plasmid

   #187972

   Purpose

   TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. bRA90

   Plasmid

   #100951

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDUP3

   Plasmid

   #92174

   Purpose

   Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

   Depositor

   Judith Berman

   Article

   Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

   Insert

   NAT1
   

   Use

   Genbank: u03440.1

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-mTID-HA3-HIS3MX6

    Plasmid

    #227983

    Purpose

    To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    miniTurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PHBA02

    Plasmid

    #83536

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUNIV-EGFP

    Plasmid

    #24706

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Insert

    EGFP (eGFP Aequoria victoria)
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Gal1/10p CAS9 Gal1/10p I-SceI

    Plasmid

    #182519

    Purpose

    Galactose-Induced Expression vector for Cas9 and I-SceI.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Inserts

    I-SceI
    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. bRA66

    Plasmid

    #100952

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTCW012

    Plasmid

    #83527

    Purpose

    Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RSV-betaGal

    Plasmid

    #24058

    Depositor

    Herbert Samuels, Frederick Stanley

    Article

    Flug et al J Biol Chem. 1987 May 5. 262(13):6373-82

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Tags

    RSV promoter

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-SENP8m

    Plasmid

    #18717

    Depositor

    Edward Yeh

    Article

    Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

    Insert

    FLAG-SENP8m (SENP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C153A

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pJZC603

    Plasmid

    #62317

    Purpose

    sgRNA with 2x PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x PP7 RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. csr-1_son-1_mTagBFP2

    Plasmid

    #191746

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Use

    Tags

    NCU04502 promoter and mTagBFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge