We narrowed to 4,353 results for: erf

1. pLminP_Luc2P_RE57

   Plasmid

   #90402

   Purpose

   Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   GR

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-FIP3

   Plasmid

   #74753

   Purpose

   Expressed sfGFP-tagged FIP3 in E. coli for protein production

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab11 family interacting protein 3 (RAB11FIP3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167935

   Purpose

   Inducible CRISPRi expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   KRAB-dCas9-DHFR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSP3545-dCas9Str

   Plasmid

   #153516

   Purpose

   Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   dCas9Str
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   nisA

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX_311-KRAB-dCas9

   Plasmid

   #96918

   Purpose

   for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

   Depositor

   John Doench, William Hahn, David Root

   Article

   Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   dCas9

   Promoter

   EF1a

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac dCas9-KRAB

   Plasmid

   #191314

   Purpose

   piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   KRAB-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ153

   Plasmid

   #69304

   Purpose

   Gateway entry vector with pco-dCas9-3X(SRDX)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-3X(SRDX) (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   2XFLAG

   Expression

   Mutation

   SRDX (X3) repressor fusion; NLS fusion

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRG2.1

   Plasmid

   #108098

   Purpose

   Lentiviral expression plasmid of sgRNA with GFP

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4xHRE_YB TATA-sfGFP-CMV_dsRed

    Plasmid

    #117399

    Purpose

    sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_dCas9-KRAB-MeCP2_Blast

    Plasmid

    #221501

    Purpose

    Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    dCas9-KRAB-MeCP2-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Use

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available Since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Use

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hUbC-dCas9 KRAB-T2A-GFP

    Plasmid

    #67620

    Purpose

    Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 KRAB T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW91.dCas9

    Plasmid

    #104372

    Purpose

    Expresses dCas9

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE TRE dCas9

    Plasmid

    #50915

    Purpose

    Tet-regulatable dCas9 lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMJ841

    Plasmid

    #39318

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    T7

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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