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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

Plasmid

#126705

Purpose

donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

GFP

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f F366H

Plasmid

#204141

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Phenylalanine 366 changed to histidine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-151

Plasmid

#118565

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-flex-iGABASnFR2-WPRE

Plasmid

#218877

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSKA413

Plasmid

#80381

Purpose

Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

ccaS (complement)
ccaR (complement)
gfpmut3

Use

Synthetic Biology; Optogenetics

Tags

FLAG tag

Expression

Bacterial

Promoter

ccaR and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-161

Plasmid

#118573

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-161

Plasmid

#118541

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-141

Plasmid

#118557

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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