We narrowed to 13,228 results for: sequence
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Plasmid#159742PurposeExpresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYOG_P2A_Hygro_Barcode
Plasmid#120465PurposeBarcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b- mKate2-KDEL-pA
Plasmid#109494PurposeMuscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequenceDepositorInsertactc1b- mKate2-KDEL-pA
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
WNK2 K207A
Plasmid#24570DepositorAvailable SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-mCherry
Plasmid#174473PurposeExpress mCherry with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertmCherry with golgi apparatus-targeting sequence
Tagsβ1,4-galactose transferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-mCherry
Plasmid#175841PurposeExpress mCherry with nucleus-targeting sequence in mammalian cellsDepositorInsertmCherry with nucleus-targeting sequence
TagsH2B (human histone 2B)ExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
TagsFirst 8 codons of COX3MutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-CEN_ARS_HIS
Plasmid#202129PurposeLevel 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.DepositorInsertCEN_ARS_HIS
UseSynthetic BiologyMutationARS sequence mutated to remove sapI site for dome…Available SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…ExpressionBacterialPromoterEscherichia coli lacIN promoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SIX1_P2A_Hygro_Barcode
Plasmid#120476PurposeBarcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only