We narrowed to 29,740 results for: REP

1. pDEST40-2XFL-Src-S4-UD-KD

   Plasmid

   #140301

   Purpose

   Expresses Flag-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   lacks the SH3 and SH2 domains

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBB0405-TEV-His12

   Plasmid

   #137040

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRevA-TEV-His12

   Plasmid

   #137034

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAF07416.1 (revA Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pP13-TEV-His12

   Plasmid

   #137035

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN432

   Plasmid

   #137870

   Purpose

   Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPN431

   Plasmid

   #137869

   Purpose

   Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPN435

   Plasmid

   #137867

   Purpose

   Expression of gRNA i3 targeting TCF4 for CRISPRi; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPN434

   Plasmid

   #137866

   Purpose

   Expression of gRNA i2 targeting TCF4 for CRISPRi; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPN446

   Plasmid

   #137868

   Purpose

   Expression of gRNA a1 targeting TCF4 for CRISPRa; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPN454

    Plasmid

    #137865

    Purpose

    Expression of gRNA i1 targeting TCF4 for CRISPRi; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKI-cjACTB-EGFP-3'short

    Plasmid

    #129749

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPRIME-CMV-GFP-shCrh2

    Plasmid

    #132710

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shCrh(2) (Crh Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKI-cjACTB-EGFP-5'short

    Plasmid

    #129748

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKI-cjACTB-EGFP

    Plasmid

    #129747

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

    Plasmid

    #105022

    Purpose

    Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Apc (Apc Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

    Plasmid

    #105028

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

    Plasmid

    #105029

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

    Plasmid

    #105035

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

    Plasmid

    #105017

    Purpose

    Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Kcmf1 (Kcmf1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIE-N-hα2M-H6

    Plasmid

    #128493

    Purpose

    Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (S24-E908) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pS6-N-hα2M-H6

    Plasmid

    #128496

    Purpose

    Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-A788) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3_nmPKA-CαN-mPAGFP

    Plasmid

    #114130

    Purpose

    Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA3_PKA-Cα(S11D)-mPAGFP

    Plasmid

    #114135

    Purpose

    Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3_PKA-Cα(S11A)-mPAGFP

    Plasmid

    #114134

    Purpose

    Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3_PKA-CαN-mPAGFP

    Plasmid

    #114114

    Purpose

    Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLL5 sgRNA(MS2)-MS2-hA3a D131A

    Plasmid

    #112131

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131A

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131A mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D131A mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482-GFP

    Plasmid

    #108500

    Purpose

    inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746iPRC1 304-509 (PRC1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha Helix, insert PRC1 microtubule domain

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP

    Plasmid

    #108503

    Purpose

    inducible expression of vsv-INCENP d543-746-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746 (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha helix

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5-FRT-TO-vsv-Incenp d543-746iPRC1_279-482-GFP

    Plasmid

    #108504

    Purpose

    inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746iPRC1 304-509 (PRC1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha Helix, insert PRC1 microtubule domain

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP

    Plasmid

    #108505

    Purpose

    inducible expression of vsv-INCENP 8KR-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    K548R, K563R, K602R, K609R, K614R, K622R, K630R, …

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge