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Showing: 8551 - 8600 of 9374 results

  1. mEmerald-CCPB1-N-18

    Plasmid
    #54028
    Purpose
    Localization: Actin Capping Protein , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CCPB1
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pET12a-JOH1

    Plasmid
    #47098
    Depositor
    Marina Ramirez-Alvarado
    Insert
    JOH1 (IGKV3-20 Human)
    Tags
    ompT
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pET12a-DAH

    Plasmid
    #47096
    Depositor
    Marina Ramirez-Alvarado
    Insert
    DAH (IGKV3-20 Human)
    Tags
    ompT
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pOpen-ALPI

    Plasmid
    #165555
    Purpose
    CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    CIP (calf intestinal phosphatase)
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165555-plasmid-map-sequence-id-339907

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  6. mCherry-CCPB1-N-18

    Plasmid
    #55010
    Purpose
    Localization: Actin Capping Protein , Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CCPB1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. mCherry-CCPB1-C-18

    Plasmid
    #55009
    Purpose
    Localization: Actin Capping Protein , Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CCPB1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    John Doench, David Root
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

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  9. N2c(dG)-hChR2-mCherry

    Plasmid
    #73681
    Purpose
    Expresses channelrhodopsin fused to mCherry for monosynaptic circuit mapping and optogenetic activation
    Depositor
    Thomas Jessell
    Insert
    hChR2-mCherry
    Use
    Neurotropic virus
    Mutation
    H134R in hChR2
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. mEos3.2-CCPB1-N-18

    Plasmid
    #57449
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    CCPB1
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57449-plasmid-map-sequence-id-216841

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  12. tdEos-CCPB1-C-18

    Plasmid
    #57591
    Purpose
    Localization: Actin Capping Protein , Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    CCPB1
    Tags
    tdEos
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

    Plasmid
    #86451
    Purpose
    Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)
    Depositor
    Gregor Bucher
    Insert
    Hsp_p-GAL4Delta-SV40;attp
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86451-plasmid-map-sequence-id-171105

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  14. pENTR ARRET

    Plasmid
    #111384
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET
    Use
    Gateway entry cloning
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111384-plasmid-map-sequence-id-209223

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  15. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7.AviTag
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  16. PS-CFP2-MCPB2-N-18

    Plasmid
    #57239
    Purpose
    Localization: Mannosidase/Golgi, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    MCPB2
    Tags
    PS-CFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 13, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    57239-plasmid-map-sequence-id-221551

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  18. pEGFP-C1-NuMA

    Plasmid
    #81029
    Purpose
    Expresses human NuMA with N-terminal EGFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    81029-plasmid-map-sequence-id-300392

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  19. pOpen-BstpolLF

    Plasmid
    #165553
    Purpose
    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst DNA Polymerase, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165553-plasmid-map-sequence-id-339898

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  20. AbVec_IGKC

    Plasmid
    #183702
    Purpose
    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGKC (IGKC )
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183702-plasmid-map-sequence-id-358531

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  21. pEYFP-C1-NuMA

    Plasmid
    #28238
    Purpose
    Expresses human NuMA with N-terminal EYFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    28238-plasmid-map-sequence-id-373778

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  22. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  23. pJK637

    Plasmid
    #72437
    Purpose
    Acetobacter aceti 1023 mutS, partial gene
    Depositor
    T. J. Kappock
    Insert
    DNA mismatch repair protein MutS (partial gene)
    Expression
    Bacterial
    Mutation
    deletes nt 1-767 and nt 2086-2655 of mutS gene
    Promoter
    n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72437-plasmid-map-sequence-id-132658

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  24. pXR004_puroR

    Plasmid
    #219820
    Purpose
    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219820-plasmid-map-sequence-id-441123

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  25. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  26. AAV-CAG-K-SPOTIT

    Plasmid
    #191488
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT
    Use
    AAV
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191488-plasmid-map-sequence-id-377866

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  27. pAV hH1-ARRET

    Plasmid
    #111374
    Purpose
    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET w/o polyA
    Use
    Adenoviral
    Promoter
    hH1
    Available Since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    111374-plasmid-map-sequence-id-209217

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  28. pLV UbC6-ARRET

    Plasmid
    #111378
    Purpose
    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET polyA
    Use
    Lentiviral
    Promoter
    UbC6
    Available Since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    111378-plasmid-map-sequence-id-209221

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  29. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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  30. pLEW-pHluorin2-PTS1

    Plasmid
    #213776
    Purpose
    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei
    Depositor
    Kenneth Christensen
    Insert
    pHlorin2
    Use
    Trypanosoma brucei expression
    Mutation
    Appended a 2 glycine linker and a T. brucei perox…
    Promoter
    T. brucei rRNA promotor
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213776-plasmid-map-sequence-id-423197

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  31. PTPRK

    Plasmid
    #38968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRK (PTPRK Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. AAV-CAG-K-red-SPOTIT

    Plasmid
    #191490
    Purpose
    Red fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-red-SPOTIT
    Use
    AAV
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191490-plasmid-map-sequence-id-377865

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  34. pENTR hH1-ARRET

    Plasmid
    #111382
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator
    Depositor
    Klaus Holzmann
    Insert
    hH1-ARRET
    Use
    Gateway entry cloning
    Promoter
    hH1
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111382-plasmid-map-sequence-id-209222

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  45. AAV-CAG-K-SPOTIT(R271F)

    Plasmid
    #191492
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT(R271F)
    Use
    AAV
    Mutation
    R271F in OR
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191492-plasmid-map-sequence-id-377952

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  47. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  49. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

    Plasmid
    #205281
    Purpose
    CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.
    Depositor
    Matthias Prigge
    Insert
    GCaMP6s
    Use
    AAV
    Available Since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205281-plasmid-map-sequence-id-412571

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Showing: 8551 - 8600 of 9374 results