We narrowed to 12,754 results for: CAR

1. pLENTI_Hu_ECT2(WT)

   Plasmid

   #136330

   Purpose

   Lentiviral expression of human ECT2 WT

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   ECT2 (ECT2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBFC0694

   Plasmid

   #186693

   Purpose

   ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsB
   tnsC
   tniQ
   Cas12k
   Sh_lacZ_α_1 gRNA
   Tn7 transposon
   GmR+sfGFP ShDART Cargo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-hygro-2xMyc-Rac1-Q61L

   Plasmid

   #128582

   Purpose

   For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Glutamine61 to Leucine.

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEAHISPAFabH

   Plasmid

   #97018

   Purpose

   Expressing Pseudomonas aeruginosa FabH protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   3-Oxoacyl-[acyl-carrier-protein] synthase III
   

   Use

   Tags

   N-ter TEV protease cleavable 6HIs

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. macroH2A2-GFP (pc2191)

   Plasmid

   #223709

   Purpose

   Expression of macroH2A2 tagged N-terminal to GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. macroH2A1.2-F192V-GFP (pc5115)

   Plasmid

   #223438

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.2-F192V (Macroh2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of phenylalanine 192 to valine (F192V)

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Puro cTnT CCND2-T2A-ChloC

   Plasmid

   #179841

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR and TALEN; Donor plasmid

   Tags

   chloride-conducting ChR (ChloC)

   Expression

   Mammalian

   Mutation

   Promoter

   Troponin T promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Puro cTnT CCND2-T2A-iChloC

   Plasmid

   #179842

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR and TALEN; Donor plasmid

   Tags

   improved chloride-conducting ChR (iChloC)

   Expression

   Mammalian

   Mutation

   Promoter

   Troponin T promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-Puro cTnT CCND2-T2A-luciferase

   Plasmid

   #179844

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR, Luciferase, and TALEN ; Donor plasmid

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Troponin T promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-851_HA-GD2-28z_HA-TFAP4

    Plasmid

    #207501

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENeCFPCNAL2 pc922

    Plasmid

    #167563

    Purpose

    For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).

    Depositor

    Cristina Cardoso

    Article

    Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

    Inserts

    Proliferating Cell Nuclear Antigen (PCNA Human)
    CFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLENTI_Hu_ECT2(T327D)

    Plasmid

    #136332

    Purpose

    Lentiviral expression of human ECT2_T327D

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    ECT2 (ECT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T327D

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA tagged SphI ELL2 M133, 138, 186ILeu

    Plasmid

    #127277

    Purpose

    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminus

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met 133, 138, 186 to Ileu; HA inserted at SphI af…

    Promoter

    pEF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTKA002

    Plasmid

    #164578

    Purpose

    Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp8
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer10b-EGFP-KRAS G12V

    Plasmid

    #164925

    Purpose

    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glycine 12 changed to valine (G->T 3156)

    Promoter

    Ubc Promoter and Ubc promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIH-TFAM-GFP

    Plasmid

    #113704

    Purpose

    Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    TFAM (Tfam Mouse)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid

    #132524

    Purpose

    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNC2H1 (DYNC2H1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker-TEV, Precission cleavag…

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CMV_ACE2-Flag_Hygro

    Plasmid

    #191998

    Purpose

    Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

    Plasmid

    #160110

    Purpose

    Expresses murine Ifih1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ifih1 (Ifih1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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