Addgene

We narrowed to 13,023 results for: Mpl

Showing: 3421 - 3440 of 13023 results

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pJH574

Plasmid

#172158

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop2); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K) PylC(E316D)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH575

Plasmid

#172159

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop3); AraC

Use

Tags

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH576

Plasmid

#172160

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop4); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K, E251D) PylD(A252V)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH19

Plasmid

#172141

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH60

Plasmid

#172142

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.CD

Use

Tags

Expression

Bacterial

Mutation

PylCD partial operon (PylB deleted to assess impa…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH77

Plasmid

#172145

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*, P83*, Y184*)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH126

Plasmid

#172147

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.SUMO-B.(SD4).CD

Use

Tags

Expression

Bacterial

Mutation

PylBCD operon (SUMO tag inserted for PylB; RBS in…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-CT-Tagging

Plasmid

#165869

Purpose

Template for amplification of mcherry C-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NT-Tagging

Plasmid

#165868

Purpose

Template for amplification of EGFP N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDM_N1_400

Plasmid

#170474

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

Insert

T7A1, O-, O1, Lambda t1 in modified lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

diminished hairpins near O1 and diminished latent…

Promoter

T7A1

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBST

Plasmid

#172968

Purpose

pWSK29 with Salmonella typhimurium wzzBST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Salmonella enterica serovar Typhimurium

Use

Tags

Expression

Bacterial

Mutation

K198E- please see depositor comments

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-fepEST

Plasmid

#172969

Purpose

pWSK29 with Salmonella typhimurium fepEST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

fepE gene from Salmonella enterica serovar Typhimurium

Use

Tags

Expression

Bacterial

Mutation

G160S- please see depositor comments

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRNA2

Plasmid

#174005

Purpose

Used to generate stable mRNA for heterologous expression experiments (no polyA)

Depositor

Stephen Ikeda

Article

Williams et al Front Neurosci. 2010 Nov 4;4:181. doi: 1

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR

Plasmid

#165043

Purpose

Repair template for CAS9 complex genome editing

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RPL13A (RPL13A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMCRW2

Plasmid

#127380

Purpose

pBC with 4 repeats of 80bp DNA template

Depositor

Richard C. Willson

Article

Chavan et al Analyst. 2020 Jul 21;145(14):4942-4949.

Insert

4 repeats of 80 bp DNA template sequence

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnt2b

Plasmid

#164964

Purpose

Zebrafish kcnt2a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt2b (LOC565691 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-FRB1C_no-linker

Plasmid

#162434

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-CFP-FRB1C_no_linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-NT-Tagging

Plasmid

#165870

Purpose

Template for amplification of mCherry N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnma1b

Plasmid

#164952

Purpose

Zebrafish kcnma1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnma1b (kcnma1b Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnmb2a

Plasmid

#164953

Purpose

Zebrafish kcnmb2a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnmb2a (kcnmb2 Zebrafish)

Use

Pcr cloning vectror

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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