We narrowed to 5,107 results for: PEP

1. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Use

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYHY3

   Plasmid

   #216109

   Purpose

   aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

   Depositor

   Shannon Sirk

   Article

   Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

   Insert

   NanoLuc
   

   Use

   Synthetic Biology; E. coli-bacteroides shuttle ve…

   Tags

   3xFLAG tag and BT3630 signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   P1TDP-A21

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-HA-USP21

   Plasmid

   #22574

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP21 (USP21 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-PTS1-IRES-Puromycin

   Plasmid

   #134665

   Purpose

   Expresses EGFP-tagged peroxisome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   tripeptide SKL (Peroxisome targeting signal 1)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE80L SpyTag-ELP-SpyTag

   Plasmid

   #112634

   Purpose

   Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   SpyTag-ELP-SpyTag
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   central cysteine

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Golgi eGFP

   Plasmid

   #79809

   Purpose

   To express and target GFP into the Golgi apparatus using lentiviruses

   Depositor

   Pantelis Tsoulfas

   Insert

   Golgi eGFP (B4GALT1 Human)
   

   Use

   Lentiviral

   Tags

   Fusion of the first 62 amino acids of the human b…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGST-PSP-hTG

   Plasmid

   #85470

   Purpose

   bacterial expression of human tissue transglutaminase

   Depositor

   Ray Truant, Jeffrey Keillor

   Article

   Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

   Insert

   hTG2 (TGM2 Human)
   

   Use

   Tags

   GST and PSP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-3C-sGFP11_pcDNA3.1(-)

   Plasmid

   #182932

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 11, FLAG tag, and HA signa…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. hPDI1_18-508_WT_pFLAG-CMV1

    Plasmid

    #31382

    Depositor

    David Ron

    Article

    Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

    Insert

    hPDI1 (P4HB Human)
    

    Use

    Tags

    FLAG M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

    Plasmid

    #131034

    Purpose

    Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRB (Tcrb Mouse)
    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-neo Flag HAUSP

    Plasmid

    #16655

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

    Insert

    HAUSP (USP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNM262

    Plasmid

    #128412

    Purpose

    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    Lysostaphin (epr Staphylococcus simulans)
    

    Use

    Synthetic Biology

    Tags

    amyE SP

    Expression

    Bacterial

    Mutation

    Promoter

    Pspank(V)

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE30-butelase1

    Plasmid

    #115430

    Purpose

    Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

    Depositor

    Joshua Mylne

    Article

    James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

    Insert

    butelase 1
    

    Use

    Tags

    6-His

    Expression

    Mutation

    Promoter

    T5

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEFIRES-P-mTagBFP-KDEL

    Plasmid

    #87163

    Purpose

    Fluorescent marker of endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    mTagBFP-KDEL
    

    Use

    Tags

    Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1 alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-GFP-P2A

    Plasmid

    #80807

    Purpose

    GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFP-P2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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