We narrowed to 4,353 results for: erf

1. pBAD-FOLD6

   Plasmid

   #186547

   Purpose

   FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   FOLD6
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

   Promoter

   araB

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-His6-GFP-C-LACE

   Plasmid

   #133913

   Purpose

   Bacterial expression of His6-GFP-C-LACE

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR59.4

   Plasmid

   #63175

   Purpose

   Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   OmpR (ompR )
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-PGK-ABI-dCas9-P2A-mcherry

   Plasmid

   #121513

   Purpose

   expression of AB1-fused-dcas9 plasmids

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   AB1 and P2A-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbS8c-ddcpf1-rfp

   Plasmid

   #153038

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQdCas9-sgempty

   Plasmid

   #159523

   Purpose

   Expression of dCas9 driven by lux cassette from Vibrio fischeri

   Depositor

   Radhakrishnan Mahadevan

   Article

   Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-PACT

   Plasmid

   #50352

   Purpose

   bacterial expression of PACT

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

   Insert

   PACT (PRKRA Human)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbS8c-ddcpf1-Δ

   Plasmid

   #153039

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti GFAABC1D-dCas9-KRAB-MeCP2

   Plasmid

   #194701

   Purpose

   Expresses dCas9-KRAB-MeCP2 in Astrocytes

   Depositor

   Timothy Jarome

   Article

   Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

   Insert

   GFAABC1D promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   None

   Promoter

   GFAABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-NED

    Plasmid

    #109358

    Purpose

    Mammalian vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    dCas9, SV40 NLS, 3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4xHRE_minCMV-sfGFP-CMV_dsRed Exp

    Plasmid

    #117401

    Purpose

    sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9+sgRNA expression vector precursor

    Plasmid

    #171798

    Purpose

    PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAct:dCas9

    Plasmid

    #78903

    Purpose

    Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB1_23_dCAS9(3xFLAG_SV40NLS)

    Plasmid

    #161478

    Purpose

    BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Insert

    dCAS9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    FLAG Tag, SV40 NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB6371

    Plasmid

    #106153

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_m4_Blasto

    Plasmid

    #167864

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-UbC-mCherry Rab5

    Plasmid

    #115239

    Purpose

    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab5a (RAB5A Human, Homo sapiens)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human UbC

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-TO-Sp dCas9-mVenusN

    Plasmid

    #191396

    Purpose

    SpdCas9 fused to N-terminal part of mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWZL Hygro-NFLAG TRF1

    Plasmid

    #16277

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa2-439

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only