Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,921 results for: pET

Clear keywords
Showing: 3421 - 3440 of 9921 results

  2. pKK-mClover3-TEV

    Plasmid
    #105778
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mClover3-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105778-plasmid-map-sequence-id-203367

    Enlarge

  3. pCIneo-hCHD7

    Plasmid
    #89458
    Purpose
    constitutive expression of human CHD7 in mammalian cells
    Depositor
    Peter Scacheri
    Insert
    CHD7 (CHD7 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89458-plasmid-map-sequence-id-176093

    Enlarge

  7. PKM2 (3GQY)

    Plasmid
    #25360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PKM2_1 (PKM Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  15. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162679-plasmid-map-sequence-id-321456

    Enlarge

  16. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162670-plasmid-map-sequence-id-321452

    Enlarge

  17. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    90473-plasmid-map-sequence-id-191118

    Enlarge

Showing: 3421 - 3440 of 9921 results