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pWJB9

Plasmid

#126650

Purpose

Expresses AdrA from a modified pLAFR3

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30

Tags

8xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-PNGT3-d2EGFP_CMV-mCherry

Plasmid

#213765

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

PNGT#3-d2EGFP

Use

Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap2a2-FKBP

Plasmid

#46944

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap2a2-FKBP

Tags

Mainly mouse Ap2a2 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pETCK3 TGP-E

Plasmid

#236255

Purpose

Expresses TGP-E, a thermostable fluorescent protein

Depositor

Natasha DeVore

Article

Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

Insert

Thermal Green Protein Q66E (TGP-E)

Tags

His tag

Expression

Bacterial

Promoter

lac operon

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSUPERIOR.puro-shp53

Plasmid

#38035

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shp53 (TP53 Human)

Use

RNAi

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pKERepPar

Plasmid

#211827

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MBII85-5'SS mut

Plasmid

#67631

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Mutation

mutation in 5'ss

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pVSL10

Plasmid

#201182

Purpose

Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

Depositor

Jack Szostak

Article

Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

Insert

Bst polymerase large fragment (BF) F710Y/D598A

Tags

HRV 3C protease cleavage site and His6

Expression

Bacterial

Mutation

F710Y D598A

Promoter

T5-lacO

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-A-TelR

Plasmid

#204979

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pZS*11_4enh

Plasmid

#149423

Purpose

Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Inserts

rbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer

Tags

T120>A

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTS2340_Tier3(SB)-iRFP_Puro

Plasmid

#169638

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven iRFP670 and PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Envelope protein

Plasmid

#215700

Purpose

Produces the Chikungunya virus envelope protein E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Dual opposing T7 promoters reporter plasmid

Plasmid

#156457

Purpose

A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Insert

NeoR/KanR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35-mut

Plasmid

#87341

Purpose

To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLC133

Plasmid

#198639

Purpose

Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

dCas9

Tags

3x-FLAG

Expression

Bacterial

Promoter

pTet

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pATmin-GG

Plasmid

#191352

Purpose

Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

Plasmid

#129708

Purpose

Express cre-dependent KASH-EGFP and scramble shRNA

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

KASH-EGFP

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

U6

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP (F64L, S65T, V163A)-TRP1

Plasmid

#53184

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pZA26

Plasmid

#158504

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMBA334

Plasmid

#214746

Purpose

High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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