We narrowed to 21,799 results for: Sis
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MT583
Plasmid#63114PurposeThe gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.DepositorInsertACD6 (Accelerated Cell Death 6)
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS_KDR
Plasmid#65667PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100E
Plasmid#65093PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to glutamic acid (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F65A
Plasmid#65092PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchange phenylalanine 65 to alanine (KDR backbone)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100A
Plasmid#65663PurposeFLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to alanine (KDR background)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F27A
Plasmid#65661PurposeFLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged phenylalanine 27 to alanine (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGMCK-0X750-luxAB-DAS+4
Plasmid#49517PurposeKanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 siteDepositorInsertP750-luxAB-DAS+4
UseTagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialMutationPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-0X750-luxAB-DAS+4
Plasmid#49519PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomallyDepositorInsertP750-luxAB-DAS+4
UseTagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialMutationPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pBAD miniSOG
Plasmid#91774PurposeBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)DepositorInsertmini Singlet Oxygen Generator
UseTagsHexa-Histidine tagExpressionBacterialMutationPromoteraraBADAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
NPY-td-Orange2
Plasmid#83497PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorInsertNPY (NPY Human)
UseTagstandem mOrange2ExpressionMammalianMutationnonePromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FLAG
Plasmid#60360PurposePlasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsEGFP and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Htr5b(3.7)-tTA-sv40
Plasmid#184412PurposeAAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.DepositorInsertMouse Htr5b gene promoter-tTA
UseAAVTagstTAExpressionMammalianMutationPromoterMouse Htr5b geneAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_G299C
Plasmid#177829PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_G299C
UseTagsExpressionMammalianMutationG299CPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
T/Brachyury-eGFP Rex Neo
Plasmid#21222DepositorInsertBrachyury promoter
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
UseTagsExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
UseTagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only
