We narrowed to 27,260 results for: Tat

1. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-sfGFP[N150TAA]

   Plasmid

   #164581

   Purpose

   superfolder GFP with N150TAA mutation

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Insert

   sfGFP-150TAA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N150TAA Mutation

   Promoter

   T7

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-GW182delta1

   Plasmid

   #11592

   Depositor

   Edward Chan

   Article

   Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

   Insert

   GW182 (TNRC6A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only aa1-498; SEE DEPOSITOR NOTES BELOW

   Promoter

   Available since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pBABE Zeo IkB SR

   Plasmid

   #83023

   Purpose

   Retroviral expression of dominant negative IkB alpha

   Depositor

   Yi Zhang

   Article

   Kim et al Cell Rep. 2016 Sep 27;17(1):275-288. doi

   Insert

   NFKB Inhibitor Alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Promoter

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK2-miR-34 MT

   Plasmid

   #78259

   Purpose

   psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   mutated miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Tags

   Expression

   Mammalian

   Mutation

   nucleotides 2-5 and 10-11 changed from CCGT to GG…

   Promoter

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. LEP-shMLL-AF9.1039

   Plasmid

   #105566

   Purpose

   retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   shRNA targeting MLL part of MLL-AF9 fusion
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSAG1::CAS9-U6::sg290860-6

   Plasmid

   #59855

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

   Depositor

   David Sibley

   Article

   Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

   Insert

   CRISPR sg290860-6
   

   Use

   CRISPR; ; toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SL2-sgTelo-MTSa/BFP/pdCas9-C1

   Plasmid

   #162760

   Purpose

   Expressing dCas9 and sgRNA containg MTSa targeting telomeres

   Depositor

   Antony K Chen

   Article

   Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

   Insert

   dCas9 and sgRNA(SL2-sgTelo-MTSa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9(nuclease deactivated Cas9)

   Promoter

   U6/CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LEP-shMLL-AF9.643

   Plasmid

   #105565

   Purpose

   retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   shRNA targeting MLL part of MLL-AF9 fusion
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-FRT-TO-GFP-PALB2 KR

    Plasmid

    #71107

    Purpose

    mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    first 8 Lysine residues mutated to Arginine (K14-…

    Promoter

    CMV/TO

    Available since

    Dec. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPotef-cdc3CB16-NatR

    Plasmid

    #86465

    Purpose

    Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

    Depositor

    Michael Feldbrügge

    Article

    Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

    Insert

    Cdc3
    

    Use

    Ustilago maydis expression

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Potef

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. ALIX(P801G)-Strep

    Plasmid

    #180024

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX(1-868, P801G)-Strep (PDCD6IP Human)
    

    Use

    Tags

    Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-)_mtZFN(-)2G

    Plasmid

    #62799

    Purpose

    mtZFN2G architecture bearing (-) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(-)2G
    

    Use

    Tags

    Internal FLAG tag immediately after F1B mitochond…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(-)_mtZFN(+)2G

    Plasmid

    #62798

    Purpose

    mtZFN2G architecture bearing (+) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(+)2G
    

    Use

    Tags

    Internal HA tag immediately after F1B mitochondri…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJH4687

    Plasmid

    #200783

    Purpose

    Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    ins-22 (ins-22 Nematode)
    

    Use

    Tags

    pHiuorin

    Expression

    Worm

    Mutation

    Promoter

    Pnpr-4

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

    Plasmid

    #204975

    Purpose

    Co-express EGFP and SARS-CoV-2 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTY152 EGFP-T2A-ORF6 SARS-CoV-1

    Plasmid

    #204974

    Purpose

    Co-express EGFP and SARS-CoV-1 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2ADW-lox-mCherry-hyBRET-ERK-NLS

    Plasmid

    #170290

    Purpose

    For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    lox-mCherry-lox-hyBRET-ERK-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO HSPH1

    Plasmid

    #19453

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPH1 (HSPH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJCC_075 AsCas12a D908A

    Plasmid

    #171668

    Purpose

    For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a D908A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 908 to alanine

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BAS285 C terminal mKO2 hygBR

    Plasmid

    #74080

    Purpose

    S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    mKO2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pUD628

    Plasmid

    #103018

    Purpose

    expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crADE2-3.S (ADE2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Hy_pMT dati

    Plasmid

    #91800

    Purpose

    Expresses the Drosophila dati circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

    Insert

    dati (dati Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

    Plasmid

    #191394

    Purpose

    R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1 R44Q-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R44Q

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJR2956

    Plasmid

    #122533

    Purpose

    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenLox_U6 Nlgn2

    Plasmid

    #59358

    Purpose

    Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

    Depositor

    Peter Scheiffele

    Article

    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

    Inserts

    Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Mouse, Rat)
    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and mouse U6

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GluR2_adRNA(1,20,6)-ADAR2

    Plasmid

    #124620

    Purpose

    Expresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    ADAR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3/FLHIP1

    Plasmid

    #29587

    Depositor

    Theodora Ross

    Article

    Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

    Insert

    Huntingtin-interacting protein 1 (HIP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA5FRT/TO-p97wt-mycStrep

    Plasmid

    #31837

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pJH3456

    Plasmid

    #179581

    Purpose

    Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    BFP

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1

    Available since

    March 2, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. SFG.CNa12_opt.IRES.eGFP

    Plasmid

    #22490

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin A mutant 12 (PPP3CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    T351E; L354A

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pESC-LEU-PmCDA1

    Plasmid

    #60818

    Purpose

    to overexpress lamprey deaminase in yeast

    Depositor

    Youri Pavlov

    Article

    Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

    Insert

    CDA1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSiren_EGFP-shHmga1#3

    Plasmid

    #122289

    Purpose

    Knockdown for mouse Hmga1

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Knockdown shRNA for mouse Hmga1 (Hmga1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. plko.1/shMCT4 477

    Plasmid

    #99598

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pIRESpuro2-UBXD1-3x HA

    Plasmid

    #31836

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    UBXD1 (UBXN6 Human)
    

    Use

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3.2/V5 mmu-mir-223

    Plasmid

    #26334

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-223 (Mir223 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA3.2/V5 hsa-mir-142

    Plasmid

    #26309

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-142 (MIR142 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

40. CS2 Flag-Smad2 (100-467)

    Plasmid

    #14928

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (100-467) (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    amino acids 100-467

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only