Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 20,363 results for: ren

Showing: 6881 - 6920 of 20363 results

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pAFMW-Nbr[K284A]

Plasmid

#188595

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K284A [AAG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A]

Plasmid

#188594

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K187A]

Plasmid

#188593

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K187A [AAA=> GCA]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr

Plasmid

#188584

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KBB430

Plasmid

#185091

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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KBB428

Plasmid

#185090

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1-myc

Plasmid

#185511

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

deletion of IDILGE in acidic blob

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1

Plasmid

#185510

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

deletion of IDILGE in acidic blob

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB4

Plasmid

#185513

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

IDILGE replaced with AAAAAA

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB2

Plasmid

#185512

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

IDIL replaced with AAAAAA

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaAB-myc

Plasmid

#185503

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

acidic blob (aa 21-33) deleted

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaAB

Plasmid

#185502

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

acidic blob (aa 21-33) deleted

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-QR-myc

Plasmid

#185521

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

replaces Q>R upstream of GARQYLNI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-QR

Plasmid

#185520

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

replaces Q>R upstream of GARQYLNI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-LA-myc

Plasmid

#185519

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

replaces L>A upstream of GARQYLN

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-LA

Plasmid

#185518

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

replaces L>A upstream of GARQYLNI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-GARP-myc

Plasmid

#185517

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

Replaces Q to P in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-GARP

Plasmid

#185516

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

Replaces Q to P in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-GARG-myc

Plasmid

#185515

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

Replaces Q to G in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-GARG

Plasmid

#185514

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

Replaces Q to G in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-F>E-myc

Plasmid

#185509

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Myc

Mutation

F>E

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-deltaRII-Cterm

Plasmid

#185504

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

RII domain and C-term deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-deltaN-myc

Plasmid

#185500

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

N-terminus deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-deltaC-myc

Plasmid

#185501

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

C-terminus deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-A6-myc

Plasmid

#185507

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

FSIENIM to AAAAAAM

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α-woolly-monkey-retroCHMP3(148)-HA

Plasmid

#154250

Purpose

expresses prematurely truncated woolly monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-pygmy-marmoset-retroCHMP3(148)-HA

Plasmid

#154251

Purpose

expresses prematurely truncated pygmy marmoset retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Flag-stm2585

Plasmid

#174397

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK129-stm2585_S130A

Plasmid

#174403

Purpose

Bacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Mutation

sarA with S130A mutation

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK129-stm2585_Y167F

Plasmid

#174402

Purpose

Bacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Mutation

sarA with Y167F mutation

Promoter

Endogenous

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Flag-gp130dimer-stm2585

Plasmid

#174395

Purpose

Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain and sarA chimera

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Flag-stm2585

Plasmid

#174391

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Tags

Cmyc and HA

Expression

Yeast

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLyGo-Bs-4

Plasmid

#163142

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLyGo-Ec-4

Plasmid

#163130

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLyGo-Bs-3

Plasmid

#163141

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLyGo-Bs-2

Plasmid

#163140

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLyGo-Ec-3

Plasmid

#163129

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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