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Showing: 8601 - 8650 of 9374 results

  1. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

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  9. mEos3.2-CCPB1-C-18

    Plasmid
    #57448
    Purpose
    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    CCPB1 (CAPZB Chicken)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

    Plasmid
    #231805
    Purpose
    Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.
    Depositor
    Annette Oxenius
    Insert
    eGFP-mCEP83
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231805-plasmid-map-sequence-id-460689

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  12. AAV-CAG-K-red-SPOTIT(R271F)

    Plasmid
    #191494
    Purpose
    Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-red-SPOTIT(R271F)
    Use
    AAV
    Mutation
    R271F in OR
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191494-plasmid-map-sequence-id-377895

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  13. Venus-BclXL-ActA-pEGFP-C1

    Plasmid
    #177410
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177410-plasmid-map-sequence-id-348171

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  14. mCerulean3-BclXL-ActA-s2193

    Plasmid
    #177411
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    Human ferritin
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177411-plasmid-map-sequence-id-347943

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  15. mCerulean3-BclXL-Cb5-s2193

    Plasmid
    #177413
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    Human ferritin
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177413-plasmid-map-sequence-id-347944

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  16. mCerulean3-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177414
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177414-plasmid-map-sequence-id-348199

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  17. Venus-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177415
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177415-plasmid-map-sequence-id-348201

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  18. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177419
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177419-plasmid-map-sequence-id-348305

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  19. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177422
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177422-plasmid-map-sequence-id-353685

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  26. PS-CFP2-CCPB1-C-18

    Plasmid
    #57215
    Purpose
    Localization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    CCPB1 (CAPZB Chicken)
    Tags
    PS-CFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 13, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    57215-plasmid-map-sequence-id-218706

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  30. pDB5050

    Plasmid
    #171126
    Purpose
    A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.
    Insert
    OsTIR1
    Use
    Other
    Mutation
    non applicable
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171126-plasmid-map-sequence-id-341097

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  31. pAAV2-CAGIG-WGA-mCherry

    Plasmid
    #224119
    Purpose
    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.
    Depositor
    Pantelis Tsoulfas
    Insert
    WGA-mCherry
    Use
    AAV
    Tags
    WGA and mCherry
    Expression
    Mammalian
    Mutation
    All mutations related to the WGA in this construc…
    Promoter
    CAGIG
    Available Since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    224119-plasmid-map-sequence-id-446520

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  33. CAPZA1-TwinStrepII

    Plasmid
    #83193
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CAPZA1 (Capza1 Mouse)
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83193-plasmid-map-sequence-id-163489

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  34. 4RRE

    Plasmid
    #64662
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    See comments
    Available Since
    May 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. 4ROH

    Plasmid
    #64659
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH:JMC081-G03:C229184 (ITCH Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. PS-CFP2-Actin-C-18

    Plasmid
    #57214
    Purpose
    Localization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Tags
    PS-CFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57214-plasmid-map-sequence-id-192846

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  40. mCerulean3-BclXL-ActA-pEGFP-C1

    Plasmid
    #177412
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177412-plasmid-map-sequence-id-348192

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  41. mCerulean3-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177418
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177418-plasmid-map-sequence-id-348297

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  42. mCerulean3-Bcl2-ActA-s2193

    Plasmid
    #177420
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    Human ferritin
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177420-plasmid-map-sequence-id-358832

    Enlarge

  43. mCerulean3-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177421
    Purpose
    Express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177421-plasmid-map-sequence-id-348306

    Enlarge

  44. mCerulean3-Bcl2-cb5-s2193

    Plasmid
    #177423
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    Human ferritin
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177423-plasmid-map-sequence-id-347946

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  47. pHH0103 ITCH WW domain #3

    Plasmid
    #104218
    Purpose
    Bacterial expression of WW domain #3 from ITCH
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ITCH WW domain #3 (ITCH Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104218-plasmid-map-sequence-id-204297

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  48. pHH0103 ITCH WW domain #4

    Plasmid
    #104219
    Purpose
    Bacterial expression of WW domain #4 from ITCH
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ITCH WW domain #4 (ITCH Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104219-plasmid-map-sequence-id-204288

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  49. pHH0103 ITCH WW domain #1

    Plasmid
    #104216
    Purpose
    Bacterial expression of WW domain #1 from ITCH
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ITCH WW domain #1 (ITCH Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104216-plasmid-map-sequence-id-204198

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Showing: 8601 - 8650 of 9374 results