Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 35,392 results for: CaS;

Showing: 3441 - 3460 of 35392 results

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px335-sgRNA-EF(Cas9n)-Kdm1a-R

Plasmid

#122328

Purpose

Expresses sgRNA targeting mouse Kdm1a and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Kdm1a

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ncapd3-L

Plasmid

#122329

Purpose

Expresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ncapd3

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-L2

Plasmid

#122307

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1 (Hdac1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-R2

Plasmid

#122308

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHes1-L

Plasmid

#122309

Purpose

Expresses sgRNA targeting mouse Hes1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hes1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHes1-R

Plasmid

#122310

Purpose

Expresses sgRNA targeting mouse Hes1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hes1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Peg3-L

Plasmid

#122311

Purpose

Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Peg3

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Peg3-R

Plasmid

#122312

Purpose

Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Peg3

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Rad21-L

Plasmid

#122313

Purpose

Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rad21

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Rad21-R

Plasmid

#122314

Purpose

Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rad21

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ssrp1-L

Plasmid

#122315

Purpose

Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ssrp1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ssrp1-R

Plasmid

#122316

Purpose

Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ssrp1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-SpCas9-HF4-2A-EGFP-L2

Plasmid

#141049

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF4-2A-EGFP

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-SpCas9-HF2-2A-EGFP-L2

Plasmid

#141050

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF2-2A-EGFP

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone3-12 (OptiHF-SpCas9)

Plasmid

#131737

Purpose

Expresses OptiHF-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

OptiHF-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

Q695A+K848A+E923M+T924V+Q926A

Promoter

EFS

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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U6-FnCas12a-TLR-MCV1-gRNA

Plasmid

#117412

Purpose

FnCas12a-gRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

FnCas12a gRNA targeting TLR 2.0

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS-Hypa-nCas9n-A69

Plasmid

#125645

Purpose

micro-injection of poly-adenylated Hypa-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

Hypa-nCas9n

Use

Rna in vitro transcription vector

Mutation

N692A, M694A, Q695A, H698A from wild-type Cas9

Promoter

T3

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFGRNA-LbCpf1-spCas9-MS2

Plasmid

#119675

Purpose

Empty vector for cloning LbCpf1-spCas9 MS2-fgRNA

Depositor

Yongsub Kim

Article

Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp-rastvTEV-teTVMV2

Plasmid

#116098

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp-rastvTEV-teTVMV2

Expression

Mammalian

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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