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pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

Plasmid

#101062

Purpose

AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2-H396K

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV CAG ChR2 E123T T159C 2A tDimer

Plasmid

#85399

Purpose

High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E123T , T159C

Promoter

CAG and ribosomal skip sequence 2A

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

Plasmid

#209781

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

cTNT

Use

AAV

Tags

HA and OLLAS

Expression

Mutation

Promoter

cTNT

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

Plasmid

#209782

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

sgRNA

Use

AAV

Tags

HA and OLLAS

Expression

Mutation

Promoter

cTNT

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

Plasmid

#107704

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3-P2A-CheRiff

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135978

Purpose

gRNA against the right loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA2

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Expression

Mutation

Promoter

CB

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135979

Purpose

gRNA against the left loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA1

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Expression

Mutation

Promoter

U6

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCR6-eLOV-TEVcs-tTA

Plasmid

#173116

Purpose

Expresses CCR6 receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

Plasmid

#186044

Purpose

AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

NES-jGCaMP8f

Use

AAV

Tags

NES-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114103

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

motoneuron-specific Homeobox 9 promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CaMKII-TVA-P2A-N2cG

Plasmid

#172362

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

Plasmid

#153538

Purpose

AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

EF1α1.1

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-mCherry

Plasmid

#35508

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

Plasmid

#102986

Purpose

AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

membrane-targeted SNAP protein after FLP-mediated recombination
Nuclear-targeted eGFP after FLP-mediated recombination

Use

AAV; Adeno associated viral vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

Plasmid

#102935

Purpose

An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

NK1R (Tacr1 Rat)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(Luc)-GFP

Plasmid

#197887

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

Plasmid

#178585

Purpose

Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

DLX2 (DLX2 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

Plasmid

#32483

Purpose

Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

ChR2HA-2a-PSAML141F,Y115F:GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

ChR2 2A

Expression

Mutation

Promoter

CAG

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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