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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJB038 pAAV EF1a flox(mCherry)-EGFP

Plasmid

#252771

Purpose

An AAV vector that expresses a Cre-dependent mCherry to eGFP

Depositor

Joshua Leonard

Article

Boucher et al J Extracell Vesicles. 2026 Apr;15(4):e70

Insert

flox(mCherry)-EGFP

Use

AAV

Promoter

hEF1a

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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AAV Syn-coca-5HT3-IRES-mCherry

Plasmid

#242197

Purpose

AAV vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-coca-5HT3-IRES-mCherry (Htr3a Mouse)

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

Synapsin

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

Plasmid

#216276

Purpose

AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Promoter

human synapsin 1 (hSyn)

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

Plasmid

#141236

Purpose

AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox; Cre-off

Tags

6xHIS tag and nuclear export signal

Promoter

human elongation factor-1 alpha (EF-1 alpha)

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-eGFP

Plasmid

#247941

Purpose

AAV transfer plasmid encoding enhanced Green Fluoresence Protein control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Oliveras-Salva et al Mol Neurodegener. 2013 Nov 25;8:44. doi:

Insert

eGFP

Use

AAV

Promoter

CMVenh synapsin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-EcYtR(NGS-6)-mCherry

Plasmid

#217363

Purpose

Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA mutant, "NGS-6"

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

Plasmid

#201686

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1 (V106W)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

Plasmid

#153532

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

Plasmid

#74306

Purpose

Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Inserts

TRPV4 (Trpv4 Rat)
ferritin (FTL and HTL1) (FTL, FTH1 Human)

Use

AAV and Cre/Lox

Tags

FLAG and p2A

Expression

Mammalian

Promoter

CMV (after cre recombination)

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

Plasmid

#230988

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Cre-dependent, membrane-localized optopatch for neuronal dendrites

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGria3

Plasmid

#124855

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

Plasmid

#124860

Purpose

Mutagenesis of Slc17a6

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

Plasmid

#124861

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

Plasmid

#114377

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C-linker-TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-HM4Di-YFP

Plasmid

#209193

Purpose

Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

Depositor

Larry Zweifel

Article

Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

Insert

Hm4Di

Use

AAV

Tags

YFP

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2

Plasmid

#195526

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR-Kv2.1PR

Plasmid

#195525

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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