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pET28b-SpRY-His

Plasmid

#179320

Purpose

Plasmid for bacterial expression and purification of SpRY-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpRY

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag YES1

Plasmid

#20665

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

YES1 (YES1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PRKACB

Plasmid

#20596

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKACB (PRKACB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PDK1

Plasmid

#20564

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PDK1 (PDK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag HK2

Plasmid

#20501

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK2 (HK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PLK1

Plasmid

#20589

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PTK2

Plasmid

#20610

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PTK2 (PTK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PKN2

Plasmid

#20587

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKN2 (PKN2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-YFP-Rb-IRES-blasticidin

Plasmid

#223964

Purpose

Fluorescent reporter with exogenous Rb

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Delta [N151/3.3R]

Plasmid

#114515

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Delta (Rat). Derived from hybridoma N151/3.3.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Delta (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabrd Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.puro_shGLS

Plasmid

#110335

Purpose

shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS glutaminase (GLS Synthetic, Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-hGDNF

Plasmid

#122035

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

hGDNF (GDNF Human)

Use

AAV

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKAA1

Plasmid

#20595

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKAA1 (PRKAA1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pT3-EF1A-CCNE1

Plasmid

#162553

Purpose

Overexpression of CCNE1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE1 (CCNE1 Human)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MVK

Plasmid

#20616

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MVK (MVK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
Anti-Kv4.2 K+ Channel (External) [K57/1R]

Plasmid

#128622

Purpose

Mammalian Expression Plasmid of anti-Kv4.2 K+ Channel (External) (Human). Derived from hybridoma K57/1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv4.2 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCND2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel (External) [K39/25R]

Plasmid

#128620

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (External) (Human). Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCNB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-LRRTM4 [N205B/22R]

Plasmid

#128636

Purpose

Mammalian Expression Plasmid of anti-LRRTM4 (Human). Derived from hybridoma N205B/22.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-LRRTM4 (Human) recombinant mouse monoclonal antibody (LRRTM4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PAK4

Plasmid

#20460

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PAK4 (PAK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only