We narrowed to 87,390 results for: lias
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pCS2+PIF6CreN
Plasmid#131780PurposeMammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite systemDepositorInsertPIF6CreN
UseCre/Lox; Zebrafish expressionTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt-AH
Plasmid#39533DepositorInsertAkt (PH domain only) (Akt1 Mouse)
UseTags6G linker and EGFPExpressionMammalianMutationPH domain onlyPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES
Plasmid#69910PurposeExpresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-YdLight1
Plasmid#125705PurposeExpresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.DepositorInsertYdLight1
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL188
Plasmid#22277PurposeExpresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertTiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-DR-cr in PBCSK-
Plasmid#126640PurposeCloning vector AsCas12a DR-crRNA for expression in mammalian cellsDepositorInsertAsCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH6
Plasmid#86555PurposeSubcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiencyDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR; SubcloningTagsExpressionMutationPromoterArabidopsis U6-26 promoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) HIPK3 Sense
Plasmid#60634PurposeExpresses the human HIPK3 exon 2 circular RNADepositorInsertHomeodomain-interacting protein kinase 3 (HIPK3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
optoPKAW196R/Y204A
Plasmid#104546PurposeExpresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.DepositorInsertCry2-mCherry-PKA W196R/Y204A
UseTagsmCherryExpressionMammalianMutationPKA W196R and Y204APromoterCMVAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA (pcDNA3-FLAG)
Plasmid#35062DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsFLAG and HisExpressionMammalianMutationContains HinA domain: aa 192-393PromoterCMVAvailable sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(515-710)-HinB (pcDNA3-FLAG)
Plasmid#35063DepositorInsertIFI16-(515-710)-HinB (IFI16 Human)
UseTagsFLAGExpressionMammalianMutationContains HinB domain: aa 515-710PromoterCMVAvailable sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFH6_new
Plasmid#105866Purposemore efficient version of pFH6; Subcloning of any sgRNA via BbsI siteDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR and Synthetic Biology; SubcloningTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-Ift52
Plasmid#128875PurposeFor mammalian expression of mouse Ift52DepositorInsertIft52 (Ift52 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G'
Plasmid#209124PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-G'DepositorInsertSUMO-A'-l4-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 (RNAi-res)
Plasmid#136645PurposeExpression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1 (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTCPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-hTRAF3 1-448
Plasmid#44033DepositorInsertTNF receptor-associated factor 3 (TRAF3 Human)
UseTags1xHAExpressionMammalianMutationContains amino acids 1-448; V274D and S393GPromoterAvailable sinceApril 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKMD106h - intein-Cas9(C574)
Plasmid#64191PurposeExpresses intein-Cas9(C574) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cGR2176
Plasmid#188258PurposePlasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcGR2176
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_HEK4_20-7
Plasmid#201974PurposeExpress a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology armDepositorInsertpegRNA_HEK4_20-7
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only
