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Plasmid#44485DepositorInsertMinimal Mos1, PuroR, peel-1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-011
Plasmid#78699PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
IG703: pMVP (L1-R5) TRE promoter
Plasmid#121697PurposepMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTRE promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-009
Plasmid#78696PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
XW83
Plasmid#65841PurposeMosSCI-Pmig-13::QF::SL2::mCherryDepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsmCherryExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Nnmt in situ probe
Plasmid#45631DepositorInsertNnmt in situ probe (Nnmt Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 454-908 of NM_010924.2PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-TuDmiR122 seedsupplemental
Plasmid#35668DepositorInsertTuD miR-122seedsupplemental
UseTagsExpressionMutationPromoterU6Available sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1000
Plasmid#44475DepositorInsertMinimal Mos1, PuroR, peel-1, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAVscCBPI TuDmiR122Gpluc7xlet7BT
Plasmid#35648DepositorInsertTuD miR-122
UseAAVTagsExpressionMutationPromoterU6Available sinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAVscCBPITuDlet-7Gpluc7x122BT
Plasmid#35650DepositorInsertTuD let-7
UseAAVTagsExpressionMutationPromoterU6Available sinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMS19
Plasmid#229475PurposeExpresses RssB with E135A, E136A, & E137A mutationDepositorInsertRssB (E135A, E136A, E137A)
UseTagsExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS21
Plasmid#229476PurposeExpresses RssB with E135A, E136A, & E137A mutation with His-tagDepositorInsertRssB (E135A, E136A, E137A)
UseTags10xHisExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
UseTags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only